Summary of 115-Nabeshima-DuodenumVilliCell

SSBD:database
SSBD:database URL
Title
Lineage trace images of LacZ cells in intestinal villi of the duodenum at different days
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
3 ( Image datasets: 3, Quantitative data datasets: 0 )
Size of Datasets
681.0 KB ( Image datasets: 681.0 KB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
transgene expression
Molecular Function (MF)
Biological Process (BP)
DNA methylation, embryogenesis
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
WideField
Contrast method
Darkfield
Microscope model
Olympus FV1000D
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Ryo Nabeshima, Osamu Nishimura, Takako Maeda, Natsumi Shimizu, Takahiro Ide, Kenta Yashiro, Yasuo Sakai, Chikara Meno, Mitsutaka Kadota, Hidetaka Shiratori, Shigehiro Kuraku, Hiroshi Hamada (2018) Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters., eLife, Volume 7

Published in 2018 Aug 2 (Electronic publication in Aug. 2, 2018, midnight )

(Abstract) We have examined the role of Fam60a, a gene highly expressed in embryonic stem cells, in mouse development. Fam60a interacts with components of the Sin3a-Hdac transcriptional corepressor complex, and most Fam60a(-/-) embryos manifest hypoplasia of visceral organs and die in utero. Fam60a is recruited to the promoter regions of a subset of genes, with the expression of these genes being either up- or down-regulated in Fam60a(-/-) embryos. The DNA methylation level of the Fam60a target gene Adhfe1 is maintained at embryonic day (E) 7.5 but markedly reduced at E9.5 in Fam60a(-/-) embryos, suggesting that DNA demethylation is enhanced in the mutant. Examination of genome-wide DNA methylation identified several differentially methylated regions, which were preferentially hypomethylated, in Fam60a(-/-) embryos. Our data suggest that Fam60a is required for proper embryogenesis, at least in part as a result of its regulation of DNA methylation at specific gene promoters.
(MeSH Terms)

Contact
Hiroshi Hamada , RIKEN , RIKEN Center for Biosystems Dynamics Research , Laboratory for Organismal Patterning
Contributors
Ryo Nabeshima, Osamu Nishimura, Takako Maeda, Natsumi Shimizu, Takahiro Ide, Kenta Yashiro, Yasuo Sakai, Chikara Meno, Mitsutaka Kadota, Hidetaka Shiratori, Shigehiro Kuraku, Hiroshi Hamada


Dataset List of 115-Nabeshima-DuodenumVilliCell

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 4676
Dataset Kind Image data
Dataset Size 205.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4677
Dataset Kind Image data
Dataset Size 244.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4678
Dataset Kind Image data
Dataset Size 231.7 KB
4D view
SSBD:OMERO
Download BDML
Download Image data