Detail of fig2H_tamoxifen_day3
Project
Title
confocal microscopy image of LacZ cells in intestinal villi of the duodenum at day 3 after injection of tamoxifen in adult mice harboring a Fam60a-CreERT2 transgene and lacZ reporter gene.
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
244.1 KB
Datatype
transgene expression
Molecular Function (MF)
Biological Process (BP)
embryogenesis
(
GO:0009790
)
DNA methylation
(
GO:0006306
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
-
T scale
-
Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
WideField
Contrast method
Darkfield
Microscope model
Olympus FV1000D
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Nabeshima et al. (2018) eLife, 7: e36435.
Related paper(s)
Ryo Nabeshima, Osamu Nishimura, Takako Maeda, Natsumi Shimizu, Takahiro Ide, Kenta Yashiro, Yasuo Sakai, Chikara Meno, Mitsutaka Kadota, Hidetaka Shiratori, Shigehiro Kuraku, Hiroshi Hamada (2018) Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters., eLife, Volume 7
Published in 2018 Aug 2 (Electronic publication in Aug. 2, 2018, midnight )
- doi: 10.7554/eLife.36435
-
pmid: 30070635
(Abstract) We have examined the role of Fam60a, a gene highly expressed in embryonic stem cells, in mouse development. Fam60a interacts with components of the Sin3a-Hdac transcriptional corepressor complex, and most Fam60a(-/-) embryos manifest hypoplasia of visceral organs and die in utero. Fam60a is recruited to the promoter regions of a subset of genes, with the expression of these genes being either up- or down-regulated in Fam60a(-/-) embryos. The DNA methylation level of the Fam60a target gene Adhfe1 is maintained at embryonic day (E) 7.5 but markedly reduced at E9.5 in Fam60a(-/-) embryos, suggesting that DNA demethylation is enhanced in the mutant. Examination of genome-wide DNA methylation identified several differentially methylated regions, which were preferentially hypomethylated, in Fam60a(-/-) embryos. Our data suggest that Fam60a is required for proper embryogenesis, at least in part as a result of its regulation of DNA methylation at specific gene promoters.(MeSH Terms)
- Animals
- DNA Methylation/genetics[major]
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics[major]
- Embryonic Development/genetics[major]
- Gene Expression Regulation, Developmental
- Genome
- Histone Deacetylases/chemistry
- Histone Deacetylases/genetics
- Mice
- Mice, Knockout
- Promoter Regions, Genetic
- Repressor Proteins/chemistry
- Repressor Proteins/genetics
- Sin3 Histone Deacetylase and Corepressor Complex
Contact
Hiroshi Hamada
, RIKEN
, RIKEN Center for Biosystems Dynamics Research
, Laboratory for Organismal Patterning
Contributors
Ryo Nabeshima, Osamu Nishimura, Takako Maeda, Natsumi Shimizu, Takahiro Ide, Kenta Yashiro, Yasuo Sakai, Chikara Meno, Mitsutaka Kadota, Hidetaka Shiratori, Shigehiro Kuraku, Hiroshi Hamada
OMERO Dataset
OMERO Project
Source