SSBD:database

292-Katsuta-StressFiber

Author reviewed

SSBD:OMERO.gallery SSBD:OMERO

SSBD:downloads

Project ID

292-Katsuta-StressFiber

Project SID

292

Title

Description

Contractile force generated in actomyosin stress fibers (SFs) is transmitted along SFs to the extracellular matrix (ECM), which contributes to cell migration and sensing of ECM rigidity. In this study, the authors show that efficient force transmission along SFs relies on actin crosslinking by alpha-actinin. Upon reduction of alpha-actinin-mediated crosslinks, the myosin II activity induced flows of actin filaments and myosin II along SFs, leading to a decrease in traction force exertion to ECM. The fluidized SFs maintained their cable integrity probably through enhanced actin polymerization throughout SFs. A computational modeling analysis suggested that lowering the density of actin crosslinks caused viscous slippage of actin filaments in SFs and, thereby, dissipated myosin-generated force transmitting along SFs. As a cellular scale outcome, alpha-actinin depletion attenuated the ECM-rigidity-dependent difference in cell migration speed, which suggested that alpha-actinin-modulated SF mechanics is involved in the cellular response to ECM rigidity.

Submission Date

2024-04-17

Opened Date

Release Date

2025-04-10

Update Date

Kind

Image datasets and Quantitative datasets

License

CC BY

Project URL

Project DOI

Metadata Version

Template Version

Funding Information

This study was supported by the grant for collaborative research between Nagoya University and R-Pharm (2614Dj-02b to M.S.), JSPS KAKENHI (20K12596 to H.H., 21H05127 to H.H., 22H05170 to S.O., 21H03804 to K.N., 22K19890 to K.N., 19H01147 to M.K., 22KK0145 to M.K., and 21K18326 to S.K.), JST CREST (JPMJCR1921 to S.O.), AMED (21bm0704065h0002 to S.O.), and Nagoya University Kyosaidan.

Total

2356 files / 34.5 GB

Image datasets

zipped 125 files / 13.6 GB, raw image 2231 files / 20.9 GB, total 2356 files / 34.5 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Biosample

Organism

Mus musculus ( NCBITaxon:10090 )

Strain

Cell

Cell line

C2C12 cell ( BTO:0000165 )

MeSH

( D018613, D059008 )

Stress Fibers

( D036681, D059008 )

( D018625 )

Ontology

Anatomical Entity

UBERON

Biological Process

regulation of stress fiber assembly ( GO:0051492 )

actin nucleation ( GO:0045010 )

cell migration ( GO:0016477 )

Cellular Component

cytoskeleton, stress fiber ( GO:0001725 )

cell surface ( GO:0009986 )

Molecular Function

actin filament binding ( GO:0051015 )

Medical Subject Headings

( D018613, D059008 )

Stress Fibers

( D036681, D059008 )

( D018625 )

Imaging Method

Method involved in biological imaging

confocal microscopy ( FBbi:00000251 )

time lapse microscopy ( FBbi:00000249 )

FRAP ( FBbi:00000366 )

atomic force microscopy ( FBbi:00000259 )

Paper DOI / Paper URL

PMID: 36852278 , doi: 10.1016/j.isci.2023.106090

People

Contact

Hiroaki Hirata

Kanazawwa Institute of Technology

Imaging dataset contributor

Hiroki Katsuta (Okayama University)

Satoru Okuda (Kanazawa University)

Kazuaki Nagayama (Ibaraki University)

Hiroaki Machiyama (Tokyo Medical University)

Hiroaki Hirata (Kanazawwa Institute of Technology)

Quantitative dataset contributor

Hiroki Katsuta (Okayama University)

Image datasets

125

Quantitative datasets

Dataset List

Thumbnail

Dataset

Organism

Kind / Links

Fig1E_Video2_ctrl_1

Mus musculus ( NCBITaxon:10090 )