292-Katsuta-StressFiber/VideoS1_ctrl
Author reviewedTime-lapse images of LifeAct-mCherry and myosin IIA-GFP in non-target (control) cells
Project
Dataset ID
VideoS1_ctrl
Kind
Image data
Title
Time-lapse images of LifeAct-mCherry and myosin IIA-GFP in non-target (control) cells
Description
Time-lapse images of LifeAct-mCherry and myosin IIA-GFP in non-target (control) cells, showing the dynamics of stress fiber. Channel1: myosin IIA-GFP, Channel2: LifeAct-mCherry
Folder Name
VideoS1_ctrl
File Format
.nd2
File Size
244MB
File Separation Method
-
Figure Number
VideoS1
Dimensions
1024x1024x1x2x61
X Scale
0.15 micrometer/pixel
Y Scale
0.15 micrometer/pixel
Z Scale
-
T Scale
1 minute
Channel
2 channel
Quantitative Data
-
Biosample
Description
C2C12 cells expressing myosin IIA-GFP and LifeAct-mCherry with non-target shRNA
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
-
Cell line
C2C12 cell (BTO:0000165)
Intrinsic Variable
-
Extrinsic Variable
C2C12 cells expressing myosin IIA-GFP and LifeAct-mCherry with non-target shRNA
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
regulation of stress fiber assembly (GO:0051492)
Cellular Component
cytoskeleton (GO:0005856)
stress fiber (GO:0001725)
Molecular Function
actin filament binding (GO:0051015)
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
gene knock out
Gene
MYH2
Protein / Probe
Myosin-2
Method / Genotype
-
Gene
-
Protein / Probe
-LifeAct
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GFP, mCherryFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Body
A1Rsi, Nikon
Module
-
Light Source
-
Detector
-
Objective
Plan Apo, Nikon
Filter Set
-
Dichroic
-