SSBD:database

292-Katsuta-StressFiber/Fig5D_Video5_ctrl thumbnail

292-Katsuta-StressFiber/Fig5D_Video5_ctrl

Author reviewed
Time-lapse images of actin-GFP FRAP on an stress fibers in non-target C2C12 cells
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Project
292-Katsuta-StressFiber
Dataset ID
Fig5D_Video5_ctrl
Kind
Image data
Title
Time-lapse images of actin-GFP FRAP on an stress fibers in non-target C2C12 cells
Description
Fluorescence recovery after photobleaching (FRAP) experiments of non-target C2C12 cells. Stress fibers in C2C12 cells expressing GFP-tagged actin were locally photobleached with 405 nm laser for 0.5 sec with 100 percent of the laser power (the size of bleached area was 1.6 x 3.6 mm), and fluo- rescence images were acquired every 0.5 sec for 1 min with 3-10 percent of the laser power.
Folder Name
Fig5D_Video5_ctrl
File Format
.nd2
File Size
15MB
File Separation Method
-
Figure Number
Fig5D
Dimensions
256x256x1x1x122
X Scale
0.224 micrometer/pixel
Y Scale
0.224 micrometer/pixel
Z Scale
-
T Scale
0.5 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
C2C12 cells expressing GFP-actin with non-target shRNA
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
-
Cell line
C2C12 cell (BTO:0000165)
MeSH
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
C2C12 cells expressing GFP-actin with non-target shRNA

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
actin nucleation (GO:0045010)
Cellular Component
cytoskeleton (GO:0005856)
stress fiber (GO:0001725)
Molecular Function
actin filament binding (GO:0051015)
MeSH Anatomy
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)
MeSH BP
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)
MeSH CC
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)
MeSH MF
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
gene knock out
Gene
-
Protein / Probe
-LifeAct
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
FRAP (FBbi:00000366)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Fluorescence Recovery After Photobleaching (D036681)
Stress Fibers (D022502)
Time-Lapse Imaging (D059008)

Instrument

Body
A1Rsi, Nikon
Module
-
Light Source
-
Detector
-
Objective
Plan Apo, Nikon
Filter Set
-
Dichroic
-