SSBD:database

274-Aini-MitochondrialDyn/Figure3A_PC3_Prophase thumbnail

274-Aini-MitochondrialDyn/Figure3A_PC3_Prophase

Author reviewed
Fluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by PC3.
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
274-Aini-MitochondrialDyn
Dataset ID
Figure3A_PC3_Prophase
Kind
Image data
Title
Fluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by PC3.
Description
Dynamic mitochondrial distribution during prophase of rice zygotes, visualized by PC3 (N-aryl pyrido cyanine 3), a newly developed N-aryl PC deriative, which is good for observation of mitochodrial dynamics staining in H2B-GFP-expressing zygotes.
Folder Name
Figure3A_PC3_Prophase
File Format
.tif
File Size
3.2 MB
File Separation Method
-
Figure Number
Figure3Ab
Dimensions
1024x1024x1x3x1
X Scale
0.208 micrometer
Y Scale
0.208 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
-

Biosample

Description
The rice zygotes were stained by PC3. The mitochondria during the prophase of rice zygotes were observed.
Organism
Oryza sativa temperate japonica subgroup (NCBITaxon:1736657)
Strain
-
Cell
zygote (CL:0010017)
Cell line
-
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)
Intrinsic Variable
-
Extrinsic Variable
H2B-GFP was expressed in rice zygotes. PC3 was applied to cells to stain mitochondria.

Ontology

Anatomical Entity
-
UBERON
zygote stage (UBERON:0000106)
Biological Process
mitochondrion distribution (GO:0048311)
Cellular Component
mitochondrion (GO:0005739)
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)
MeSH BP
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)
MeSH CC
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)
MeSH MF
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
-PC3
Method / Genotype
staining
Gene
-
Protein / Probe
H2B
Method / Genotype
transfection

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
probe for mitochondria GFP
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Mitochondria (D008928)
Cell Nucleus (D002467)

Instrument

Body
Zeiss LSM 710
Module
-
Light Source
a laser scanning confocal microscope with 543-nm excitation wavelengths and>560-nm emission wavelengths
Detector
-
Objective
-
Filter Set
-
Dichroic
-