274-Aini-MitochondrialDyn/Figure3B_MitoTrackerRed_Prophase
Author reviewedFluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by MitoTrackerRed.
Project
Dataset ID
Figure3B_MitoTrackerRed_Prophase
Kind
Image data
Title
Fluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by MitoTrackerRed.
Description
Dynamic mitochondrial distribution during prophase of rice zygotes stained with MitoTracker Red in H2B-GFP-expressiong zygotes.
Folder Name
Figure3B_MitoTrackerRed_Prophase
File Format
.tif
File Size
3.2 MB
File Separation Method
-
Figure Number
Figure3Bb
Dimensions
1024x1024x1x3x1
X Scale
0.132 micrometer
Y Scale
0.132 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
-
Biosample
Description
The rice zygotes were stained by MitoTracker Red. The mitochondria during the prophase of rice zygotes were observed.
Organism
Oryza sativa temperate japonica subgroup (NCBITaxon:1736657)
Strain
-
Cell
zygote (CL:0010017)
Cell line
-
Intrinsic Variable
-
Extrinsic Variable
H2B-GFP was expressed in rice zygotes. MitoTracker Red was applied to cells to stain mitochondria.
Ontology
Anatomical Entity
-
UBERON
zygote stage (UBERON:0000106)
Biological Process
mitochondrion distribution (GO:0048311)
Cellular Component
mitochondrion (GO:0005739)
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-MitoTracker Red
Method / Genotype
staining
Gene
-
Protein / Probe
H2B
Method / Genotype
transfection
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
probe for mitochondria
GFP
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Body
Zeiss LSM 710
Module
-
Light Source
a laser scanning confocal
microscope with 543-nm
excitation wavelengths and>560-nm emission wavelengths
Detector
-
Objective
-
Filter Set
-
Dichroic
-