77-Aoki-CellDynERK
Author reviewedProject name
77-Aoki-CellDynERK
Release date
2018-11-14
Update date
2022-11-21
Pre-release URL
-
Project title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
License
Method summary
See details in Aoki et al. (2017) Dev Cell, 43(3): 305-317.
PubMed URL
Kind
Image data
Quantitative data
Organism
Canis lupus familiaris
(
NCBITaxon:9615
)
Total
37 files / 1.7 GB
Image datasets
zipped 15 files / 336.5 MB, raw image 19 files / 1.4 GB, total 34 files / 1.7 GB
Quantitative datasets
zipped 1 files / 34.7 KB, raw bdml 2 files / 548.0 KB, total 3 files / 582.8 KB
Image datasets
14
Quantitative datasets
1
Strain
MDCK
Datatype
cell dynamics
Basedon
Experiment
XYZ-scale
XY: 1.48 micrometer/pixel, Z: NA
XY: 0.91 micrometer/pixel, Z: NA
XY: 0.49 micrometer/pixel, Z: NA
XY: 0.65 micrometer/pixel, Z: NA
T-scale
180 second for each time interval
120 second for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
-
Filter set
-
Biological Process
activation of MAP ERK kinase kinase
(
GO:0000186
)
Cellular Component
-
People
Contact
Kazuhiro Aoki
National Institutes of Natural Sciences
National Institute for Basic Biology
Division of Quantitative Biology
5-1, Higashiyama, Myodaiji, Okazaki, Aichi 444-8787 Japan
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of ERK activity during the wound-healing process in MDCK cells expressing EKAREV-NLS.
Time-lapse images of the clockwise rotation of ERK activation on a circular-patterned substrate and counterclockwise rotation of cell migration during the wound-healing process in MDCK cells expressing EKAREV-NLS.
Time-lapse images of ERK activation induce collective cell migration through the regulation of the MLC.
Time-lapse images of the spontaneous wave of ERK activation in MDCK cells expressing EKAREV-NLS and MCL-mCherry.
Time-lapse images of the accumulations of MCL-mCherry in MDCK cells expressing EKAREV-NLS and MCL-mCherry.
Time-lapse images of the spontaneous wave of ERK activation and the accumulation of MCL-mCherry.
BDML file for quantiative information about MLC-mCherry accumulation in MDCK cells expressing EKAREV-NLS and MLC-mCherry.
Time-lapse images of the spontaneous wave of ERK activation in MDCK cells expressing EKAREV-NLS.
Time-lapse images of the H2B-iRFP in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Time-lapse images of the mCherry-ERK in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Montage images of the optic flow of cell migration in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Time-lapse epifluorescence images of spontaneous waves in MDCK cells expressing EKAREV-NLS with the presence of ADAM10 inhibitor.
Time-lapse epifluorescence images of spontaneous waves in MDCK cells expressing EKAREV-NLS treated with DMSO.
Time-lapse epifluorescence images of light-induced ERK acivation in MDCK cells expressing EKAREV-NLS co-cultured with MDCK cells expressing CRY2-CRaf, CIBN-EGFP-KRasCT, and H2B-iRFP.
Time-lapse epifluorescence images of light-induced ERK acivation in MDCK cells expressing EKAREV-NLS co-cultured with MDCK cells expressing CRY2-CRaf, CIBN-EGFP-KRasCT, and H2B-iRFP.