Dataset 77-Aoki-CellDynERK/Figure5A
Author reviewed
Project title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Contact name
Kazuhiro Aoki
Organization
National Institutes of Natural Sciences
Department
National Institute for Basic Biology
Laboratory
Division of Quantitative Biology
Address
5-1, Higashiyama, Myodaiji, Okazaki, Aichi 444-8787 Japan
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
Method summary
See details in Aoki et al. (2017) Dev Cell, 43(3): 305-317.
Title
Time-lapse images of the spontaneous wave of ERK activation in MDCK cells expressing EKAREV-NLS.
XYZ-scale
XY: 1.48 micrometer/pixel, Z: NA
T-scale
180 second for each time interval
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.41
Filter set
FF01-438/24(Semrock, Rochester, NY);FF01-542/27(Semrock)
Biological Process
activation of MAP ERK kinase kinase
(
GO:0000186
)
Sibling records
No sibling records.