Dataset 77-Aoki-CellDynERK/Figure6C_opticflow
Author reviewedProject name
Release date
2018-11-14
Update date
2022-11-21
Pre-release URL
None
Project title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Contact name
Kazuhiro Aoki
Organization
National Institutes of Natural Sciences
Department
National Institute for Basic Biology
Laboratory
Division of Quantitative Biology
Address
5-1, Higashiyama, Myodaiji, Okazaki, Aichi 444-8787 Japan
License
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
Method summary
See details in Aoki et al. (2017) Dev Cell, 43(3): 305-317.
PubMed URL
File or Folder
Figure6C_opticflow
Kind
Image data
Title
Montage images of the optic flow of cell migration in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Description
-
Organism
Canis lupus familiaris
(
NCBITaxon:9615
)
Strain
MDCK
Datatype
cell dynamics
Basedon
Experiment
LocalID
Figure6C_opticflow
XYZ-scale
XY: 1.48 micrometer/pixel, Z: NA
T-scale
120 second for each time interval
dblink
None
External links
-
Source
None
BDML
None
PDPML
None
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.44
Filter set
FF01-641/75(Semrock);FF01-692/LP(Semrock)
Sample
-
Dye
-
Clearing
-
Note0
-
Biological Process
activation of MAP ERK kinase kinase
(
GO:0000186
)
Cellular Component
-
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Sibling records
No sibling records.