37-Arata-MolDynPar2
Author reviewedProject name
37-Arata-MolDynPar2
Release date
2017-10-03
Update date
2020-02-03
Pre-release URL
-
Project title
-
License
Method summary
See details in Arata et al. (2016) Cell Reports, 16, 1-13.
The original move of pmPAR-2 (tiff format) is rotated at 135 degree and processed through Rolling Ball and Kalman filters to obtain pmPAR-2_RBKL.avi (Movie S3). The PAR-2 trajectories (xlsx file) were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.
The original move of wtPAR-2 (tiff format) is rotated at 45 degree and processed through Rolling Ball and Kalman filters to obtain wtPAR-2_RBKL.avi (Movie S1). The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.
The original move of wtPAR-2/pkc-3 (tiff format) is rotated at 135 degree and processed through Rolling Ball and Kalman filters to obtain wtPAR-2pkc-3_RBKL.avi (Movie S2). The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.
PubMed URL
Kind
Image data
Quantitative data
Organism
Caenorhabditis elegans
(
NCBITaxon:6239
)
Total
24 files / 2.6 GB
Image datasets
zipped 3 files / 1.1 GB, raw image 3 files / 1.5 GB, total 6 files / 2.6 GB
Quantitative datasets
zipped 6 files / 2.0 MB, raw bdml 12 files / 32.6 MB, total 18 files / 34.6 MB
Image datasets
3
Quantitative datasets
3
Strain
-
Datatype
single molecule dynamics
Basedon
Experiment
XYZ-scale
XY: 0.0667 micrometer/pixel, Z: NA
T-scale
0.05 second for each time interval
dblink
None
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
Nikon TE2000
Detector model
Hamamatsu Photonics ImagEM
Objective model
Nikon Apo TIRF 60X/1.49
Filter set
Dichroic mirror: DM495 Nikon, Emission filter: BA500-545 Nikon
Biological Process
cellular protein localization
(
GO:0034613
)
Cellular Component
-
People
Contact
Yukinobu Arata, Yasushi Sako
RIKEN
Wako
Cellular Informatics Laboratory
2-1, Hirosawa, Wako, Saitama 351-0198 Japan
Contributors
Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W. Wiseman, Hitoshi Sawa, Tetsuya J. Kobayashi, Hugo B. Brandão, Tatsuo Shibata, Yasushi Sako
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::phospho-mimicPAR-2 in a C. elegans wild-type embryo
BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::phospho-mimicPAR-2 in a C. elegans wild-type embryo
Time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 expressed in a C. elegans wild-type embryo
BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 expressed in a C. elegans wild-type embryo
Time-lapse semi-TIRF microscopy images of Fluorescent particles of GFP::PAR-2 in a C. elegans pkc-3(RNAi) embryo
BDML file of the PAR2 trajectories obtained from time-lapse semi-TIRF microscopy images of Fluorescent particles of GFP::PAR-2 in a C. elegans pkc-3(RNAi) embryo