Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W. Wiseman, Hitoshi Sawa, Tetsuya J. Kobayashi, Hugo B. Brandão, Tatsuo Shibata, Yasushi Sako
Method summary
The original move of wtPAR-2 (tiff format) is rotated at 45 degree and processed through Rolling Ball and Kalman filters to obtain wtPAR-2_RBKL.avi (Movie S1). The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.
BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 expressed in a C. elegans wild-type embryo