Methods summary
See details in Arata et al. (2016) Cell Rep. 16, 1-13. The original move of pmPAR-2 (tiff format) is rotated at 135 degree and processed through Rolling Ball and Kalman filters to obtain pmPAR-2_RBKL.avi. The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.