Summary of 47-Noguchi-CellDyn
Kind
Image data
based on Experiment
Number of Datasets
38
( Image datasets: 38,
Quantitative data datasets: 0 )
Size of Datasets
90.2 GB
( Image datasets: 90.2 GB,
Quantitative data datasets: 0 bytes )
Gene symbol(s)
Ascl1, Ret
Protein name(s)
CXCR4, H2B, Laminin, N-cadherin, PGP9.5, RET
Protein tag(s)
EGFP, mCherry
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.828 micrometer/pixel, Z: 7.0 micrometer/pixel, XY: 1.656 micrometer/pixel, Z: 11.9 micrometer/pixel, XY: 0.414 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 0.489 micrometer/pixel, Z: 3.0 micrometer/pixel, XY: 0.033 micrometer/pixel, Z: 0.375 micrometer/pixel, XY: 0.828 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.636 micrometer/pixel, Z: 7.0 micrometer/pixel, XY: 1.136 micrometer/pixel, Z: 6.5 micrometer/pixel, XY: 1.656 micrometer/pixel, Z: 17.179 micrometer/pixel, XY: 0.196 micrometer/pixel, Z: NA, XY: 0.132 micrometer/pixel, Z: NA, XY: 0.830 micrometer/pixel, Z: NA, XY: 0.664 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 0.636 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.415 micrometer/pixel, Z: NA, XY: 1.325 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.461 micrometer/pixel, Z: NA, NA, XY: 0.496 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 1.325 micrometer/pixel, Z: 6.5 micrometer/pixel
Image Acquisition
Experiment type
Immunocytochemistry, Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-IX83, Carl Zeiss LSM710, Olympus FV1200MPE, NA
Detector model
NA, PMT
Objective model
Olympus XLPLN25XSVMP 25x/0.9, Carl Zeiss LD LCI Plan-Apochromat 63x/1.4 Oil DIC M27, Olympus XLPLN25XSVMP 25x/0.8, NA, Carl Zeiss Plan-Apochromat 20x/0.8 M27, Plan-Apochromat 63x/1.40 Oil DIC M27, Carl Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC M27, Olympus XLPLN25XWMP 25x/1.05, Plan-Apochromat 20x/0.8
Filter set
-
Related paper(s)
Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto (2015) Directed Migration of Pulmonary Neuroendocrine Cells toward Airway Branches Organizes the Stereotypic Location of Neuroepithelial Bodies., Cell reports, Volume 13, Number 12, pp. 2679-86
Published in 2015 Dec 29
(Electronic publication in Dec. 17, 2015, midnight )
(Abstract) The airway epithelium consists of diverse cell types, including neuroendocrine (NE) cells. These cells are thought to function as chemoreceptors and as a component of the stem cell niche as well as the cells of origin in small-cell lung cancer. NE cells often localize at bifurcation points of airway tubes, forming small clusters called neuroepithelial bodies (NEBs). To investigate NEB development, we established methods for 3D mapping and ex vivo 4D imaging of developing lungs. We found that NEBs localize at stereotypic positions in the bifurcation area irrespective of variations in size. Notch-Hes1 signaling contributes to the differentiation of solitary NE cells, regulating their number but not localization. Live imaging revealed that individual NE cells migrate distally to and cluster at bifurcation points, driving NEB formation. We propose that NEB development is a multistep process involving differentiation of individual NE cells and their directional migration to organize NEBs.
(MeSH Terms)
Contact
Masafumi Noguchi, Mitsuru Morimoto
, RIKEN
, Center for Developmental Biology
, Laboratory for Lung Development
Contributors
Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto
SSBD:OMERO.gallery Project
SSBD:OMERO Project
Dataset List of 47-Noguchi-CellDyn
#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
Dataset Kind
Image data
Dataset Size
12.8 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (8.4 MB)
Dataset Kind
Image data
Dataset Size
8.5 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (7.0 MB)
Dataset Kind
Image data
Dataset Size
757.5 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (381.2 MB)
Dataset Kind
Image data
Dataset Size
757.5 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (381.2 MB)
Dataset Kind
Image data
Dataset Size
741.5 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (368.9 MB)
Dataset Kind
Image data
Dataset Size
63.0 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (46.4 MB)
Dataset Kind
Image data
Dataset Size
22.7 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (19.2 MB)
Dataset Kind
Image data
Dataset Size
1.9 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (922.9 MB)
Dataset Kind
Image data
Dataset Size
686.0 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (381.6 MB)
Dataset Kind
Image data
Dataset Size
6.3 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (4.9 GB)
Dataset Kind
Image data
Dataset Size
5.4 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (3.7 GB)
Dataset Kind
Image data
Dataset Size
2.4 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (1.2 GB)
Dataset Kind
Image data
Dataset Size
88.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (36.3 MB)
Dataset Kind
Image data
Dataset Size
15.5 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (9.3 GB)
Dataset Kind
Image data
Dataset Size
1.7 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (960.8 MB)
Dataset Kind
Image data
Dataset Size
1.5 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (762.5 MB)
Dataset Kind
Image data
Dataset Size
45.2 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (44.4 MB)
Dataset Kind
Image data
Dataset Size
6.2 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (4.9 MB)
Dataset Kind
Image data
Dataset Size
8.5 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (7.0 MB)
Dataset Kind
Image data
Dataset Size
262.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (192.8 MB)
Dataset Kind
Image data
Dataset Size
264.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (201.5 MB)
Dataset Kind
Image data
Dataset Size
50.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (35.6 MB)
Dataset Kind
Image data
Dataset Size
916.7 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (460.3 MB)
Dataset Kind
Image data
Dataset Size
194.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (100.3 MB)
Dataset Kind
Image data
Dataset Size
2.5 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (2.4 GB)
Dataset Kind
Image data
Dataset Size
153.6 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (95.9 MB)
Dataset Kind
Image data
Dataset Size
4.2 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (4.1 GB)
Dataset Kind
Image data
Dataset Size
7.4 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (5.5 GB)
Dataset Kind
Image data
Dataset Size
5.0 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (3.4 GB)
Dataset Kind
Image data
Dataset Size
13.6 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (7.6 GB)
Dataset Kind
Image data
Dataset Size
1.8 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (1.1 GB)
Dataset Kind
Image data
Dataset Size
6.3 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (4.9 GB)
Dataset Kind
Image data
Dataset Size
3.6 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (2.7 GB)
Dataset Kind
Image data
Dataset Size
2.7 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (2.2 GB)
Dataset Kind
Image data
Dataset Size
3.2 GB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (2.6 GB)
Dataset Kind
Image data
Dataset Size
6.2 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (5.3 MB)
Dataset Kind
Image data
Dataset Size
50.3 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (43.4 MB)
Dataset Kind
Image data
Dataset Size
6.1 MB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (4.7 MB)