SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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436-Yano-MNincorporate

https://ssbd.riken.jp/database/436-Yano-MNincorporate/

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ssbd-repos-000436

https://ssbd.riken.jp/repository/436/

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In vivo imaging of micronuclei incorporation by microglia

Microglia-resident immune cells in the central nervous system-undergomorphological and functional changes in response to signals from thelocal environment and mature into various homeostatic states. However, niche signals underlying microglial differentiation and maturationremain unknown. Here, the authors show that neuronal micronuclei (MN) transfer to microglia, which is followed by changing microglial characteristics during the postnatal period. Neurons passing through a dense region of the developing neocortex give rise to MN and release them into the extracellular space, before being incorporated into microglia and inducing morphological changes. Two-photon imaging analyses have revealed that microglia incorporating MN tend to slowly retract their processes. Loss of the cGAS gene alleviates effects on micronucleus-dependent morphological changes. Neuronal MN-harboring microglia also exhibit unique transcriptome signatures. These results demonstrate that neuronal MN serve as niche signals that transform microglia, and provide a potential mechanism for regulation of microglial characteristics in the early postnatal neocortex.

2026-07-07

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CC BY 4.0

Image data based on Experiment

9 ( Image datasets: 9, Quantitative data datasets: 0 )

18.7 GB ( Image datasets: 18.7 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

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cell morphogenesis

nucleus

two-photon laser scanning microscopy, confocal microscopy

1 minute, 5 minutes

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Fuminori Tsuruta , University of Tsukuba , Institute of Life and Environmental Sciences , Institute of Life and Environmental Sciences

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