SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

Value

Search Clear/View All

---

367-Tsuboi-DrosophilaWing

https://ssbd.riken.jp/database/367-Tsuboi-DrosophilaWing/

---

ssbd-repos-000367

https://ssbd.riken.jp/repository/367/

---

The folding dynamics of Drosophila wing during pupal development

Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.

2025-11-28

-

CC BY

Image data based on Experiment

23 ( Image datasets: 23, Quantitative data datasets: 0 )

353.0 GB ( Image datasets: 353.0 GB, Quantitative data datasets: 0 bytes )

---

Drosophila

sd-Gal4/+; UAS-dpyRNAi/E-Cadher- in::GFP; UAS-mCD8::mCherry/UAS-Dcr2, sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/ UAS-NpRNAi, sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/+, Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci- Gal4/+, Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci-Gal4/ UAS-3xFLAG.dCas9.VPR, y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/ci-Gal4, ap-GAL4/UAS-dpyRNAi; UAS-Dcr2/UAS-DsRed, sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+, Dpy::YFP, Ubi-GAP43::tdTomato/+; rho^{ve-1}, vn^{1}/+, sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/Dpy::YFP, sd-Gal4/+; Dp::YFP, UAS-mCD8::mCherry/+, ap-GAL4/+; UAS-Dcr2/UAS-DsRed, y, w, hs-flp; E-Cadherin::GFP; Act > CD2 > GAL4; UAS-mCD8::mCherry, y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/hh-Gal4, sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-SbRNAi/+, Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR, Dpy::YFP, Ubi-GAP43:: tdTomato/+; rho^{ve-1}, vn^{1}/rho^{ve-1}, vn^{1}, sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/+; UAS- SbRNAi/+, sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP, Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh- Gal4/+

---

-

-

pattern specification process, pupal development

extracellular matrix, plasma membrane

confocal microscopy, time lapse microscopy, multi-photon microscopy

NA, 1 hour, 20 minutes

---

-

-

-

-

-

-

-

-

---

---

Alice Tsuboi , Kyoto University , Graduate School of Biostudies , Graduate School of Biostudies

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---

---