Summary of 276-Murai-CellularDyn

SSBD:database
SSBD:database URL
Title
Time-course images of cellular dynamics of SMART mice stimulated responds to necroptosis.
Description
-
Relase date
2024-11-25
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
23 ( Image datasets: 23, Quantitative data datasets: 0 )
Size of Datasets
276.1 MB ( Image datasets: 276.1 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
necroptotic process, execution phase of necroptosis, apoptotic process, execution phase of apoptosis
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy, FRET
T scale
2 minutes of time interval, 1 minute of time interval, 5 minutes of time interval, 1 minutes of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki, Kenta Terai, Sachiko Komazawa-Sakon, Takao Seki, Yoshifumi Yamaguchi, Tetuo Mikami, Kimi Araki, Masaki Ohmuraya, Michiyuki Matsuda, Hiroyasu Nakano (2022) Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis., Communications biology, Volume 5, Number 1, pp. 1331

Published in 2022 Dec 5 (Electronic publication in Dec. 5, 2022, midnight )

(Abstract) Necroptosis is a regulated form of cell death involved in various pathological conditions, including ischemic reperfusion injuries, virus infections, and drug-induced tissue injuries. However, it is not fully understood when and where necroptosis occurs in vivo. We previously generated a Forster resonance energy transfer (FRET) biosensor, termed SMART (the sensor for MLKL activation by RIPK3 based on FRET), which monitors conformational changes of MLKL along with progression of necroptosis in human and murine cell lines in vitro. Here, we generate transgenic (Tg) mice that express the SMART biosensor in various tissues. The FRET ratio is increased in necroptosis, but not apoptosis or pyroptosis, in primary cells. Moreover, the FRET signals are elevated in renal tubular cells of cisplatin-treated SMART Tg mice compared to untreated SMART Tg mice. Together, SMART Tg mice may provide a valuable tool for monitoring necroptosis in different types of cells in vitro and in vivo.
(MeSH Terms)

Contact
Hiroyasu Nakano , Toho University School of Medicine , Department of Biochemistry
Contributors


Dataset List of 276-Murai-CellularDyn

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Dataset ID
Kind
Size
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SSBD:OMERO
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# 11552
Dataset Kind Image data
Dataset Size 4.6 MB
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# 11553
Dataset Kind Image data
Dataset Size 18.4 MB
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SSBD:OMERO
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# 11554
Dataset Kind Image data
Dataset Size 4.6 MB
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SSBD:OMERO
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# 11555
Dataset Kind Image data
Dataset Size 20.6 MB
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SSBD:OMERO
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# 11556
Dataset Kind Image data
Dataset Size 20.6 MB
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SSBD:OMERO
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# 11557
Dataset Kind Image data
Dataset Size 6.9 MB
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SSBD:OMERO
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# 11558
Dataset Kind Image data
Dataset Size 2.3 MB
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SSBD:OMERO
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# 11559
Dataset Kind Image data
Dataset Size 3.1 MB
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SSBD:OMERO
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# 11560
Dataset Kind Image data
Dataset Size 3.1 MB
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SSBD:OMERO
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# 11561
Dataset Kind Image data
Dataset Size 3.8 MB
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SSBD:OMERO
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# 11562
Dataset Kind Image data
Dataset Size 3.8 MB
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SSBD:OMERO
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# 11563
Dataset Kind Image data
Dataset Size 5.0 MB
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SSBD:OMERO
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# 11564
Dataset Kind Image data
Dataset Size 5.0 MB
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SSBD:OMERO
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# 11565
Datast ID Fig3b_WT_BV6-zVAD
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
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# 11566
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
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# 11567
Datast ID Fig3b_WT_Untreated
Dataset Kind Image data
Dataset Size 7.3 MB
4D view
SSBD:OMERO
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# 11568
Dataset Kind Image data
Dataset Size 3.4 MB
4D view
SSBD:OMERO
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# 11569
Dataset Kind Image data
Dataset Size 10.3 MB
4D view
SSBD:OMERO
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# 11570
Dataset Kind Image data
Dataset Size 3.4 MB
4D view
SSBD:OMERO
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# 11571
Dataset Kind Image data
Dataset Size 10.3 MB
4D view
SSBD:OMERO
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# 11572
Dataset Kind Image data
Dataset Size 3.4 MB
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SSBD:OMERO
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# 11573
Dataset Kind Image data
Dataset Size 18.4 MB
4D view
SSBD:OMERO
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# 11574
Dataset Kind Image data
Dataset Size 111.6 MB
4D view
SSBD:OMERO
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