Summary of 222-Dodo-RamanImaging

SSBD:Repository
Title
Raman images of VA-13 cells or WI-38 cells treated with 100 ┬ÁM all-D-GLA at different time point
Description
-
Relase date
2022-11-23
Updated date
-
License
CC-BY-NC
Kind
Image data based on Experiment
Number of Datasets
12 ( Image datasets: 12, Quantitative data datasets: 0 )

Organism(s)
Homo sapiens
Cell lines(s)
VA-13 cell, Wi-38 cell, WI-38 cell

Datatype
-
Molecular Function (MF)
Biological Process (BP)
metabolic process
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
Image Acquisition
-
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
-

Related paper(s)

Kosuke Dodo, Ayato Sato, Yuki Tamura, Syusuke Egoshi, Koichi Fujiwara, Kana Oonuma, Shuhei Nakao, Naoki Terayama, Mikiko Sodeoka (2021) Synthesis of deuterated gamma-linolenic acid and application for biological studies: metabolic tuning and Raman imaging., Chemical communications (Cambridge, England), Volume 57, Number 17, pp. 2180-2183

Published in 2021 Mar 1

(Abstract) gamma-Linolenic acid (GLA) is reported to show tumor-selective cytotoxicity through unidentified mechanisms. Here, to assess the involvement of oxidized metabolites of GLA, we synthesized several deuterated GLAs and evaluated their metabolism and cytotoxicity towards normal human fibroblast WI-38 cells and VA-13 tumor cells generated from WI-38 by transformation with SV40 virus. Deuteration of GLA suppressed both metabolism and cytotoxicity towards WI-38 cells and increased the selectivity for VA-13 cells. Fully deuterated GLA was visualized by Raman imaging, which indicated that GLA is accumulated in intracellular lipid droplets of VA-13 cells. Our results suggest the tumor-selective cytotoxicity is due to GLA itself, not its oxidized metabolites.
(MeSH Terms)

Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi , RIKEN, RIKEN, RIKEN , Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors


Dataset List of 222-Dodo-RamanImaging

#
Dataset ID
Dataset Kind
4D View
SSBD:OMERO
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# 7773
Dataset Kind Image data
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SSBD:OMERO
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# 7774
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7775
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7776
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7777
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7778
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7779
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7780
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7781
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7782
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7783
Dataset Kind Image data
4D view
SSBD:OMERO
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# 7784
Dataset Kind Image data
4D view
SSBD:OMERO
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