SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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219-Konishi-CalciumIonDyn

https://ssbd.riken.jp/database/219-Konishi-CalciumIonDyn/

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ssbd-repos-000219

https://ssbd.riken.jp/repository/219/

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Live imaging of Ca2+ concentration dynamics in BrafV600E melanoma cells.

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2023-02-21

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CC BY

Image data based on Experiment

170 ( Image datasets: 170, Quantitative data datasets: 0 )

81.4 GB ( Image datasets: 81.4 GB, Quantitative data datasets: 0 bytes )

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Canis lupus familiaris, Mus musculus

BrafV600E cell, MDCK cell

Gnaq, Ptger1, Ptgfr, Ptgs1, Ptgs1. Ptgs2. Gnaq, Ptgs2, Tbxa2r, Tbxas1, Tbxas2r

NFAT, PKA

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G protein-coupled receptor binding

bioluminescence imaging, time lapse microscopy

0.828 micrometer/pixel, 0.637 micrometer/pixel, 0.441 micrometer/pixel, 0.454 micrometer/pixel, 0.414 micrometer/pixel, 0.638924 micrometer/pixel, 0.552 micrometer/pixel, NA

0.828 micrometer/pixel, 0.637 micrometer/pixel, 0.441 micrometer/pixel, 0.454 micrometer/pixel, 0.414 micrometer/pixel, 0.638924 micrometer/pixel, 0.552 micrometer/pixel, NA

1 micrometer/slice, NA, 5 micrometer/slice

15 sec per time interval, 3.07 sec per time interval, 6 sec per time interval, 3.08 sec per time interval, 3.02 sec per time interval, 60.22 sec per time interval, 1 min per time interval, 65.43 sec per time interval, 97.39 sec per time interval, 60.09 sec per time interval, 65.3 sec per time interval, 3.05 sec per time interval, 1 sec per time interval, 60.30 sec per time interval, 3.03 sec per time interval, 1.11 sec per time interval, 3.01 sec per time interval, 60.31 sec per time interval, 59.71 sec per time interval, 60.73 sec per time interval, 3 sec per time interval, 6.01 sec per time interval, 5.98 sec per time interval, 3.11 sec per time interval, 60.11 sec per time interval, 60.19 sec per time interval, 3.06 sec per time interval, 60.18 sec per time interval, 6.02 sec per time interval, 61.28 sec per time interval, 60.08 sec per time interval

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Yoshinobu Konishi, Hiroshi Ichise, Tetsuya Watabe, Choji Oki, Shinya Tsukiji, Yoko Hamazaki, Yasuhiro Murakawa, Akifumi Takaori-Kondo, Kenta Terai, Michiyuki Matsuda (2021) Intravital Imaging Identifies the VEGF-TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion., Cancer research, Volume 81, Number 15, pp. 4124-4132

Published in 2021 Aug 1 (Electronic publication in May 25, 2021, midnight )

• doi: 10.1158/0008-5472.CAN-20-4245
• pmid: 34035084
  

(Abstract) Prostaglandin E2 (PGE2) promotes tumor progression through evasion of antitumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE2, little is known whether PGE2 secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A2 (TXA2) triggers Ca(2+) transients in tumor cells, culminating in PGE2 secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca(2+) transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca(2+) transients were monitored as the surrogate marker of PGE2 secretion. Intravital imaging of Braf(V600E) mouse melanoma cells revealed that the proportion of cells exhibiting Ca(2+) transients is markedly higher in vivo than in vitro. The TXA2 receptor was indispensable for the Ca(2+) transients in vivo, high intratumoral PGE2 concentration, and evasion of antitumor immunity. Notably, treatment with a VEGF receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca(2+) transients and reduced TXA2 and PGE2 concentrations in tumor tissues. These results identify the VEGF-TXA2 axis as a critical promoter of PGE2-dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies. SIGNIFICANCE: This study identifies the VEGF-TXA2 axis as a potentially targetable regulator of PGE2 secretion, which provides novel strategies for prevention and treatment of multiple types of malignancies.

(MeSH Terms)

• Animals
• Dinoprostone/immunology[major]
• Humans
• Immune Evasion/immunology[major]
• Intravital Microscopy/methods[major]
• Mice
• Mice, Nude
• Vascular Endothelial Growth Factor A/immunology[major]

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Kenta Terai , kyoto University , Graduate School of Medicine , Department of Pathology and Biology of Diseases

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