Summary of 197-Bannai-CalciumIonSignaling

SSBD:database
SSBD:database URL
Title
Time-lapse imaging of local Ca2+ signals in cultured cells by membrane-targeted Ca2+ indicators
Description
-
Relase date
2022-11-23
Updated date
2023-02-16
Errata
2023/02/16 The expression "msec" changed to "millisecond"
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
7 ( Image datasets: 7, Quantitative data datasets: 0 )
Size of Datasets
894.0 MB ( Image datasets: 894.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Rattus norvegicus, Homo sapiens, Mus musculus
Strain(s)
C57/BL6J, Wistar rat
Cell lines(s)
HeLa cell
Protein name(s)
GCaMP6f, RCaMP2

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
calcium ion signaling
Cellular Component (CC)
plasma membrane
Biological Imaging Method
time lapse microscopy
X scale
0.263 micrometer/pixel, 0.229 micrometer/pixel, 0.433 micrometer/pixel
Y scale
0.263 micrometer/pixel, 0.229 micrometer/pixel, 0.433 micrometer/pixel
Z scale
-
T scale
425 milliseconds of time interval, 371 milliseconds of time interval, 575 milliseconds of time interval, 100 milliseconds of time interval, 402 milliseconds of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Hiroko Bannai, Matsumi Hirose, Fumihiro Niwa, Katsuhiko Mikoshiba (2019) Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators., Journal of visualized experiments : JoVE, Number 145

Published in 2019 Mar 22 (Electronic publication in March 22, 2019, midnight )

(Abstract) Calcium ion (Ca(2+)) is a universal intracellular messenger molecule that drives multiple signaling pathways, leading to diverse biological outputs. The coordination of two Ca(2+) signal sources-"Ca(2+) influx" from outside the cell and "Ca(2+) release" from the intracellular Ca2+ store endoplasmic reticulum (ER)-is considered to underlie the diverse spatio-temporal patterns of Ca(2+) signals that cause multiple biological functions in cells. The purpose of this protocol is to describe a new Ca(2+) imaging method that enables monitoring of the very moment of "Ca(2+) influx" and "Ca(2+) release". OER-GCaMP6f is a genetically encoded Ca(2+) indicator (GECI) comprising GCaMP6f, which is targeted to the ER outer membrane. OER-GCaMP6f can monitor Ca(2+) release at a higher temporal resolution than conventional GCaMP6f. Combined with plasma membrane-targeted GECIs, the spatio-temporal Ca(2+) signal pattern can be described at a subcellular resolution. The subcellular-targeted Ca(2+) indicators described here are, in principle, available for all cell types, even for the in vivo imaging of Caenorhabditis elegans neurons. In this protocol, we introduce Ca(2+) imaging in cells from cell lines, neurons, and glial cells in dissociated primary cultures, and describe the preparation of frozen stock of rat cortical neurons.
(MeSH Terms)

Contact
Hiroko Bannai , RIKEN , RIKEN Center for Brain Science , Laboratory for Developmental Neurobiology
Contributors


Dataset List of 197-Bannai-CalciumIonSignaling

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# 7717
Dataset Kind Image data
Dataset Size 250.6 MB
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# 7718
Dataset Kind Image data
Dataset Size 120.7 MB
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SSBD:OMERO
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# 7719
Dataset Kind Image data
Dataset Size 120.7 MB
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SSBD:OMERO
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# 7720
Dataset Kind Image data
Dataset Size 120.7 MB
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SSBD:OMERO
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# 7721
Dataset Kind Image data
Dataset Size 120.7 MB
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SSBD:OMERO
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# 7722
Dataset Kind Image data
Dataset Size 39.7 MB
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SSBD:OMERO
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# 7723
Dataset Kind Image data
Dataset Size 120.7 MB
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SSBD:OMERO
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