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Key
Organism
Strain
Cell Line
Gene Symbol
Protein name
Protein tag
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Title
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Gene Ontology
Biological Imaging Methods
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Detail of Figure3_701GOERGCaMP6fLckRCaMP2HeLax60x1His1uM
SSBD:OMERO.gallery Dataset
SSBD:OMERO Dataset
SSBD:OMERO.gallery Project
SSBD:OMERO Project
4D View for Quantitative Data
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Project
197-Bannai-CalciumIonSignaling
SSBD:Repository
ssbd-repos-000197
Title
Time-lapse imaging of HeLa cells expressing Lck-RCaMP2 and OER-GCaMP6f.
Description
Time-lapse imaging of HeLa cells expressing Lck-RCaMP2 and OER-GCaMP6f.
Release, Updated
2022-11-23,
2023-02-16
Errata in Project
2023/02/16 The expression "msec" changed to "millisecond"
License
CC BY
Kind
Image data based on NA
File Formats
.stk
Data size
250.6 MB
Organism
Homo sapiens
(
NCBI:txid9606
)
Strain(s)
-
Cell Line
HeLa cell (
CLO_0003684
)
Protein names
RCaMP2, GCaMP6f
Datatype
NA
Molecular Function (MF)
Biological Process (BP)
calcium ion signaling (
GO:0019722
)
Cellular Component (CC)
plasma membrane (
GO:0005886
)
Biological Imaging Method
time lapse microscopy (
Fbbi:00000249
)
X scale
0.433 micrometer/pixel
Y scale
0.433 micrometer/pixel
Z scale
-
T scale
100 milliseconds of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Bannai H et. al. J Vis Exp. 2019 Mar 22;(145).
Related paper(s)
Contact
Hiroko Bannai , RIKEN , RIKEN Center for Brain Science , Laboratory for Developmental Neurobiology
Contributors
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-9135
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-753
Source
https://ssbd.riken.jp/data/197-Bannai-CalciumIonSignaling/source/Figure3_701GOERGCaMP6fLckRCaMP2HeLax60x1His1uM.zip
(170.3 MB)