Hiroshi Ochiai, Takeshi Sugawara, Takashi Yamamoto (2015) Simultaneous live imaging of the transcription and nuclear position of specific genes., Nucleic acids research, Volume 43, Number 19, pp. e127
Published in 2015 Oct 30 (Electronic publication in June 19, 2015, midnight )
(Abstract) The relationship between genome organization and gene expression has recently been established. However, the relationships between spatial organization, dynamics, and transcriptional regulation of the genome remain unknown. In this study, we developed a live-imaging method for simultaneous measurements of the transcriptional activity and nuclear position of endogenous genes, which we termed the 'Real-time Observation of Localization and EXpression (ROLEX)' system. We demonstrated that ROLEX is highly specific and does not affect the expression level of the target gene. ROLEX enabled detection of sub-genome-wide mobility changes that depended on the state of Nanog transactivation in embryonic stem cells. We believe that the ROLEX system will become a powerful tool for exploring the relationship between transcription and nuclear dynamics in living cells.(MeSH Terms)