Detail of NMP-R_dCas9_MS2
Project
SSBD:Repository
Title
Time-lapse fluorescence microscopy images of mouse embryonic stem cells (NMP-R mESCs) transfected with the MS2 sigRNA expression vector of nuclease-dead Cas9 fused to the green fluorescent protein (dCas9-GFP) and MS2 coat protein fused to the tandem near-infrared red fluorescent protein (MCP-tdiRFP) signals
Description
NA
Release, Updated
2016-10-03,
2018-11-15
2018-11-15
License
CC BY-NC
Kind
Image data
based on Experiment
File Formats
Data size
4.6 MB
Protein names
dCas9, MCP
Protein tags
GFP, tdiRFP
Datatype
gene expression dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
(
GO:0034613
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.16 micrometer/pixel, Z: 0. micrometer/slice
T scale
2 minute for each time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Ochiai et al. (2015) Nucleic Acids Research, 43(19): e127
Related paper(s)
Contact
Hiroshi Ochiai
, Hiroshima University
, Research Center for the Mathematics on Chromatin Live Dynamics (RcMcD)
Contributors
Hiroshi Ochiai, Takeshi Sugiwara, Takashi Yamamoto
OMERO Dataset
OMERO Project
External Link