SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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127-Konishi-ERKDyn

https://ssbd.riken.jp/database/127-Konishi-ERKDyn/

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ssbd-repos-000127

https://ssbd.riken.jp/repository/127/

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Live-Cell FRET images and BDML quantitative information about extracellular signal-regulated kinase activity dynamics in thymocyte motility

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2021-09-30

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CC BY

Image data based on Experiment

143 ( Image datasets: 143, Quantitative data datasets: 0 )

26.4 GB ( Image datasets: 26.4 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

ERK, MHC-II

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activation of MAPERK kinase, cell motility

T cell receptor complex

time lapse microscopy, fluorescence microscopy

0.636 micrometer/pixel, 0.662 micrometer/pixel, 0.22 micrometer/pixel, 0.497 micrometer/pixel, 0.454 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.994 micrometer/pixel

0.636 micrometer/pixel, 0.662 micrometer/pixel, 0.22 micrometer/pixel, 0.497 micrometer/pixel, 0.454 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.994 micrometer/pixel

1 micrometer/slice, 2 micrometer/slice, 3 micrometer/slice

30.57 second for each time interval, 37.06 second for each time interval, 33.71 second for each time interval, 33.72 second for each time interval, 53.16 second for each time interval, 30.56 second for each time interval, 30.74 second for each time interval, 33.58 second for each time interval, 35.24 second for each time interval, NA, 1 second for each time interval, 33.47 second for each time interval, 35.32 second for each time interval, 33.96 second for each time interval, 30.93 second for each time interval, 33.61 second for each time interval, 35.35 second for each time interval, 75.3 second for each time interval, 33.87 second for each time interval, 33.63 second for each time interval, 33.64 second for each time interval, 33.57 second for each time interval, 33.59 second for each time interval, 34.8 second for each time interval, 33.83 second for each time interval, 33.7 second for each time interval, 31.01 second for each time interval, 33.88 second for each time interval, 15.01 second for each time interval, 30.53 second for each time interval, 33.73 second for each time interval, 33.55 second for each time interval, 31.77 second for each time interval, 31 second for each time interval, 40.8 second for each time interval, 33.66 second for each time interval, 33.53 second for each time interval

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Yoshinobu Konishi, Kenta Terai, Yasuhide Furuta, Hiroshi Kiyonari, Takaya Abe, Yoshihiro Ueda, Tatsuo Kinashi, Yoko Hamazaki, Akifumi Takaori-Kondo, Michiyuki Matsuda (2018) Live-Cell FRET Imaging Reveals a Role of Extracellular Signal-Regulated Kinase Activity Dynamics in Thymocyte Motility., iScience, Volume 10, pp. 98-113

Published in 2018 Dec 21 (Electronic publication in Nov. 20, 2018, midnight )

• doi: 10.1016/j.isci.2018.11.025
• pmid: 30508722
  

(Abstract) Extracellular signal-regulated kinase (ERK) plays critical roles in T cell development in the thymus. Nevertheless, the dynamics of ERK activity and the role of ERK in regulating thymocyte motility remain largely unknown due to technical limitations. To visualize ERK activity in thymocytes, we here developed knockin reporter mice expressing a Forster/fluorescence resonance energy transfer (FRET)-based biosensor for ERK from the ROSA26 locus. Live imaging of thymocytes isolated from the reporter mice revealed that ERK regulates thymocyte motility in a subtype-specific manner. Negative correlation between ERK activity and motility was observed in CD4/CD8 double-positive thymocytes and CD8 single-positive thymocytes, but not in CD4 single-positive thymocytes. Interestingly, however, the temporal deviations of ERK activity from the average correlate with the motility of CD4 single-positive thymocytes. Thus, live-cell FRET imaging will open a window to understanding the dynamic nature and the diverse functions of ERK signaling in T cell biology.

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Kenta Terai , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling

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