127-Konishi-ERKDyn
Author reviewedLive-Cell FRET images and BDML quantitative information about extracellular signal-regulated kinase activity dynamics in thymocyte motility
Project ID
127-Konishi-ERKDyn
Project DOI
-
Title
Live-Cell FRET images and BDML quantitative information about extracellular signal-regulated kinase activity dynamics in thymocyte motility
Description
-
Release Date
2021-09-30
Update Date
-
License
Version
1.0.0
Method Summary
See details in Konishi Y, et. al. (2018) iScience, 10: 98-113.
Kind
Image data
Total
310296 files / 42.4 GB
Image datasets
zipped 143 files / 16.0 GB, raw image 310153 files / 26.4 GB, total 310296 files / 42.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Paper(s)
Organism
Mus muculus
(
NCBITaxon:10090
)
Strain
-
Cell line
-
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
cell motility
(
GO:0048870
)
Cellular Component
T cell receptor complex
(
GO:0042101
)
Medical Subject Headings
-
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
143
Quantitative datasets
0
OMERO.gallery
People
Contact
Kenta Terai
Kyoto University
Graduate School of Biostudies
Laboratory of Bioimaging and Cell Signaling
Imaging dataset contributor
-
Quantitative dataset contributor
Kenta Terai
(Kyoto University)
Yoshinobu Konishi
(Kyoto University)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes overlaid on the sliced thymic lobes
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes overlaid on the sliced thymic lobes
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the motility of DP (CD4/CD8 double-positive) thymocytes in the cortex without MEKi pretreatmen
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the motility of DP (CD4/CD8 double-positive) thymocytes in the cortex without MEKi pretreatmen
Mus muculus
(
NCBITaxon:10090
)
Timelpase fluorescence images of the motility of DP (CD4/CD8 double-positive) thymocytes
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of EKAREV distribution in the intestin of Eisuke mouse through a BA 520-560 nm fileter shown in grayscale.
Fluorescence images of EKAREV distribution in the liver of Eisuke mouse through a BA 520-560 nm fileter shown in grayscale.
Fluorescence images of EKAREV distribution in the lymph of Eisuke mouse through a BA 520-560 nm fileter shown in grayscale.
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of EKAREV distribution in the lymph of EIIa-EKAREV-NES mouse through a BA 520-560 nm fileter shown in grayscale.
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of EKAREV distribution in the intestin of EIIa-EKAREV-NES mouse through a BA 520-560 nm fileter shown in grayscale.
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of EKAREV distribution in the liver of EIIa-EKAREV-NES mouse through a BA 520-560 nm fileter shown in grayscale.
Fluorescence image of T cells of the lymph node in a living mouse obtained by TPEM through a BA 520-560 nm emission filter
Mus muculus
(
NCBITaxon:10090
)
A fluorescence images of the T cells in the paracortex was injected with anti-CD3 antibody intravenously for 60 minutes
Timelapse fluorescence images of the T cells in the paracortex was injected with MEK inhibitor intravenously
Mus muculus
(
NCBITaxon:10090
)
Timelapse fluorescence images of the T cells in the paracortex before administration with anti-CD3 antibody
Fluorescence images of the thymic cortex of Lck-EKAREV-NLS mouse through a BA 520-560nm emission filter
Fluorescence images of ERK activity of the OT-I TCR (ovalbumin(OVA)-specific TCR transgenic mice ) thmocytes in the cortex showing in intesity-modulated display mode
Fluorescence images of ERK activity of the WT(Lck-EKAREV-NLS ) thmocytes in the cortex showing in intesity-modulated display mode
Mus muculus
(
NCBITaxon:10090
)
Fluorescence image of thymic lobes showing both thymocytes (BA 520-560 nm emission filter) and thymic DCs (BA 615-675 nm emission filter)
Fluorescence images of the cortex in intesity-modulated display mode
Fluorescence images of the medulla in intesity-modulated display mode
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity in cortex
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity in cortex
Fluorescence images of the ERK activity in cortex
Fluorescence images of the ERK activity in cortex
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity in medulla
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity in medulla
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity in medulla
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD8 thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated DP (CD4/CD8 double-positive) thymocytes in vitro
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes overlaid on the sliced thymic lobes
Mus muculus
(
NCBITaxon:10090
)
Fluorescence images of the ERK activity of isolated CD4 thymocytes overlaid on the sliced thymic lobes