Detail of Fig5_SDSRM
Project
SSBD:Repository
Title
Time-lapse SDSRM images of rapidly moving lysosomes stained with Lamp1-EYFP in MDCK cells
Description
NA
Release, Updated
2017-10-03,
2018-11-15
2018-11-15
License
CC BY-NC-SA
Kind
Image data
based on Experiment
File Formats
Data size
42.0 MB
Gene symbols
Lamp1
Protein tags
EYFP
Datatype
organelle dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
organelle
(
GO:0043226
)
Biological Imaging Method
XYZ Scale
XY: 100 nanometer per slice, Z: NA
T scale
0.1 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
Other
Acquisition mode
StructuredIllumination
Contrast method
Fluorescence
Microscope model
Custom made microscope system based on IX81 (Olympus) with a disk-scannning unit IX2-DSU (Olympus), custom-made disk (DU-DSR-SP; Olympus) and laser illumination optics (Sapphire488HP; Coherent)
Detector model
ORCA FLASH 4.0 v2
Objective model
Olympus UPLSAPO60XO 60x/1.3 O
Filter set
Summary of Methods
See details in Hayashi and Okada (2015) Mol Biol Cell, 26(9): 1743-1751.
Related paper(s)
Contact
Yasushi Okada
, RIKEN
, Quantitative Biology Center
, Laboratory for Cell Polarity Regulation
Contributors
Shinichi Hayashi, Yasushi Okada
OMERO Dataset
OMERO Project