Detail of fig5a_control_2
Project
SSBD:Repository
Title
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor inactive analogue.
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
6.8 MB
Protein names
Rap1
Datatype
spine dynamics
Molecular Function (MF)
Biological Process (BP)
spine apparatus formation
(
GO:1905355
)
Cellular Component (CC)
spine synapse
(
GO:0106033
)
,
neuron spine
(
GO:0044309
)
Biological Imaging Method
XYZ Scale
XY: 0.0932 micrometer/pixel, Z: 0.400 micrometer/slice
T scale
1800 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV300
Detector model
-
Objective model
PlanApo X60 1.10 Oil LSM (Olympus)
Filter set
-
Summary of Methods
See details in Koeberle et al. (2017) Sci Rep, 7(1): 13409.
Related paper(s)
Contact
Shigeo Okabe
, University of Tokyo
, Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Solveigh Cornelia Koeberle, Shinji Tanaka, Toshihiko Kuriu, Hirohide Iwasaki, Andreas Koeberle, Alexander Schulz, Dario-Lucas Helbing, Yoko Yamagata, Helen Morrison, Shigeo Okabe
OMERO Dataset
OMERO Project
Source