Detail of Fig4d_L5ET_SDL


Project
SSBD:Repository
Title
Z-series images of mouse cerebral cortex in vivo using SeeDB-Live
Description
Z-series images of anesthetized mouse cerebral cortex using SeeDB-Live. The neurons at a depth of 0-700 micrometer from the surface of the brain were imaged by two-photon confocal microscopy. EYFP expressed in the layer 5 extratelencephalic-projecting (L5ET) neurons were observed in the Thy-YFP-H transgenic mouse.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
116.6 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
1.043 micrometer
Y scale
1.043 micrometer
Z scale
3 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)

Contact
Shigenori Inagaki, Takeshi Imai , Kyushu University, Kyushu University , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors

OMERO Dataset
OMERO Project
Source