Detail of Fig3jk_NMDAstim_ctrl_3
Project
SSBD:Repository
Title
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Description
Time-lapse calcium imaging of mouse olfactory bulb slice immersed in ACSF. In the mitral cells of the olfactory bulb, GCaMP6f was expressed. Activities of the neurons stimulated with NMDA and glycine at a depth of 100 micrometer from the surface of the slice were imaged. This dataset includes the images before, during and after washing out the stimulant.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
168.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
calcium ion transport
Cellular Component (CC)
cell body
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.994 micrometer
Y scale
0.994 micrometer
Z scale
-
T scale
1.109 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source