Detail of Fig1o_CorticalOrganoid
Project
SSBD:Repository
Title
Time-lapse calcium imaging of cortical organoid using SeeDB-Live
Description
Time-lapse calcium imaging of cortical organoid induced from mouse embryonic stem cells. The organoid was immersed with SeeDB-Live during imaging. Spontaneous calcium transients of neurons were imaged with a calcium indicator, Calblyte-630AM.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
64.6 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
calcium ion transport
Cellular Component (CC)
cytoplasm
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2 micrometer
Y scale
2 micrometer
Z scale
-
T scale
0.235 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project