SSBD:database

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Detail of Fig5G_Rec8Antibody_20221220-L18

Project

421-Takenouchi-AgedYoungOocyte

SSBD:Repository

ssbd-repos-000421

Title

Time-lapse and z-series images of H2B-SNAP and tdTomato-CENPC in REC8 TRIM-Away oocytes treated with anti-REC8 antibody

Description

Time-lapse and z-series images of H2B-SNAP and tdTomato-CENPC in REC8 TRIM-Away oocytes treated with anti-REC8 antibody. By TRIM-Away method, Rec8 was knocked down by the antibody, and cohesin was partially depleted from the oocyte.

Release, Updated

2025-12-03

License

CC BY 4.0

Kind

Image data

File Formats

.tif

Data size

1.3 GB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

B6D2F1/J

Cell Line

Datatype

Molecular Function (MF)

Biological Process (BP)

female meiosis chromosome segregation ,

Cellular Component (CC)

chromosome

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )

X scale

0.119 micrometer

Y scale

0.119 micrometer

Z scale

1.5 micrometer

T scale

3 minutes

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Takenouchi O, Sakakibara Y, Kitajima TS. Live chromosome identifying and tracking reveals size-based spatial pathway of meiotic errors in oocytes. Science. 2024 Jul 19;385(6706):eadn5529.