SSBD:database

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Detail of Fig1B_Chr5Probe

Project

421-Takenouchi-AgedYoungOocyte

SSBD:Repository

ssbd-repos-000421

Title

Z-series images of mouse oocytes visualizing H2B-SNAP and chromosome5

Description

Z-series images of mouse oocytes visualizing H2B-SNAP and chromosome1. For CRISPR-Sirius method, single-guide RNAs (sgRNAs) to detect individual chromosome-specific repeat region were prepared. The repeat sequences of target chromosomes are labeled by noncleaving Cas9 (dCas9), sgRNA with aptamers, and fluorescent protein–fused aptamer-binding proteins. channel1; probe for chromosome5, channel2; H2B-SNAP, channel3; bright field

Release, Updated

2025-12-03

License

CC BY 4.0

Kind

Image data

File Formats

.tif

Data size

52.5 MB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

B6D2F1/J

Cell Line

Datatype

Molecular Function (MF)

Biological Process (BP)

Cellular Component (CC)

chromosome

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )

X scale

0.06 micrometer

Y scale

0.06 micrometer

Z scale

0.75 micrometer

T scale

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Takenouchi O, Sakakibara Y, Kitajima TS. Live chromosome identifying and tracking reveals size-based spatial pathway of meiotic errors in oocytes. Science. 2024 Jul 19;385(6706):eadn5529.