Detail of Fig4_AF_Neopetromin
Project
SSBD:Repository
Title
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of neopetromin
Description
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of neopetromin. fABD2 is C-terminal half of AtFim1 (aa 325–687), which includes the second actin-binding domain. Neopetromin is a newly found peptide from the Marine Sponge Neopetrosia sp.
Release, Updated
2025-11-28
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
80.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
actin cytoskeleton
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.185 micrometer
Y scale
0.185 micrometer
Z scale
0.5 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Hitora Y, El-Desoky AH, Sadahiro Y, Sejiyama A, Kinoshita A, Ise Y, Angkouw ED, Mangindaan REP, Higaki T, Tsukamoto S. Neopetromin, a Cyclic Tripeptide with a C-N Cross-Link, from the Marine Sponge Neopetrosia sp., That Causes Vacuole Fragmentation in Tobacco BY-2 Cells. J Nat Prod. 2024 Apr 26;87(4):1197-1202.
Related paper(s)
Yuki Hitora, Ahmed H El-Desoky, Yusaku Sadahiro, Ai Sejiyama, Amiri Kinoshita, Yuji Ise, Esther D Angkouw, Remy E P Mangindaan, Takumi Higaki, Sachiko Tsukamoto (2024) Neopetromin, a Cyclic Tripeptide with a C-N Cross-Link, from the Marine Sponge Neopetrosia sp., That Causes Vacuole Fragmentation in Tobacco BY-2 Cells., Journal of natural products
Published in 2024 Mar 19 (Electronic publication in March 19, 2024, midnight )
- doi: 10.1021/acs.jnatprod.4c00158
-
pmid: 38503712
(Abstract) HPLC-MS analysis revealed the presence of an unreported peptide in the extract of the marine sponge Neopetrosia sp. Its structure was determined as a tripeptide, named neopetromin (1), composed of two tyrosine and one tryptophan residues with a heteroaromatic C-N cross-link between side chains. The absolute configuration of amino acids was determined using Marfey's method after ozonolysis and hydrolysis of 1. Compound 1 promoted vacuole fragmentation in an actin-independent manner in tobacco BY-2 cells.
Contact
Sachiko Tsukamoto, Yuki Hitora
, Kumamoto
University, Kumamoto
University
, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences
, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences
Contributors
OMERO Project
Source