The shape of vacuole and F-actin in tobacco BY-2 cells treated with neopetromin, a newly found peptide
Description
HPLC-MS analysis revealed the presence of an unreported peptide in the extract of the marine sponge Neopetrosia sp. Its structure was determined as a tripeptide, named neopetromin (1), composed of two tyrosine and one tryptophan residues with a heteroaromatic C−N cross-link between side chains. The absolute configuration of amino acids was determined using Marfey’s method after ozonolysis and hydrolysis of 1. Compound 1 promoted vacuole fragmentation in an actin- independent manner in tobacco BY-2 cells.
negative regulation of actin filament polymerization
GO Cellular Component (CC)
vacuole, actin cytoskeleton
Study Type
NA
Imaging Methods
confocal microscopy
Method Summary
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Related paper(s)
Contact(s)
Sachiko Tsukamoto, Yuki Hitora
Organization(s)
Kumamoto University, Kumamoto University
, Department of Natural Medicines, Graduate School of Pharmaceutical Sciences, Department of Natural Medicines, Graduate School of Pharmaceutical Sciences
, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences, Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences