Detail of Fig5_retro-59



Project
SSBD:Repository
Title
BDML file for the tracking of an endosome retrogradely transported in the axons of a supracervical ganglion (SCG) neurons in vitro
Description
NA
Release, Updated
2019-11-20
License
CC BY-NC-SA
Kind
Quantitative data based on Experiment
File Formats
Data size
517.0 KB

Organism
Rattus norvegicus ( NCBI:txid10116 )
Strain(s)
-
Cell Line
-

Datatype
neuron cell dynamics
Molecular Function (MF)
Biological Process (BP)
axonal transport ( GO:0098930 )
Cellular Component (CC)
endosome ( GO:0005768 )
Biological Imaging Method
XYZ Scale
X: 1 micrometer, Y: NA, Z: NA
T scale
0.0102 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
WideField
Contrast method
Fluorescence
Microscope model
Olympus IX71
Detector model
Andor LucaS
Objective model
Olympus UPlanFL 100x/1.3
Filter set

Summary of Methods
See details in Hayashi et al. (2018) Mol Biol Cell, 29(25): 3017-3025
Related paper(s)

Kumiko Hayashi, Yuta Tsuchizawa, Mitsuhiro Iwaki, Yasushi Okada (2018) Application of the fluctuation theorem for noninvasive force measurement in living neuronal axons., Molecular biology of the cell, Volume 29, Number 25, pp. 3017-3025

Published in 2018 Dec 1 (Electronic publication in Oct. 3, 2018, midnight )

(Abstract) Although its importance is recently widely accepted, force measurement has been difficult in living biological systems, mainly due to the lack of the versatile noninvasive force measurement methods. The fluctuation theorem, which represents the thermodynamic properties of small fluctuating nonequilibrium systems, has been applied to the analysis of the thermodynamic properties of motor proteins in vitro. Here we extend it to the axonal transport (displacement) of endosomes. The distribution of the displacement fluctuation had three or four distinct peaks around multiples of a unit value, which the fluctuation theorem can convert into the drag force exerted on the endosomes. The results demonstrated that a single cargo vesicle is conveyed by one to three or four units of force production.
(MeSH Terms)

Contact
Yasushi Okada , RIKEN , Quantitative Biology Center , Laboratory for Cell Polarity Regulation
Contributors
Kumiko Hayashi, Yuta Tsuchizawa, Mitsuhiro Iwaki, Yasushi Okada

Local ID
Fig5_retro-59
BDML ID
e4aeaae5-edf4-4b20-b854-01dc22f0c0b9
BDML/BD5