Summary of 59-Yano-MicrotubuleImmuno

SSBD:database
SSBD:database URL
Title
SIM images of tubulin immunofluorescence in Eph4 cells
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
1.2 MB ( Image datasets: 1.2 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Strain(s)
Eph4
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
Microtubule immunofluorescence
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell cell junction
Biological Imaging Method
-
XYZ Scale
XY: 0.04 micrometer/pixel, Z: 0.3 micrometer/slice
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Zeiss Elyra S.1 SIM (ELYRA S.1)
Detector model
-
Objective model
Plan Apochromat 100×, 1.46 NA oil im- mersion lens, 63×, 1.4 NA oil immersion lens, and 40×, 1.4 NA oil immer- sion lens
Filter set
-

Related paper(s)

Tomoki Yano, Takeshi Matsui, Atsushi Tamura, Masami Uji, Sachiko Tsukita (2013) The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK., The Journal of cell biology, Volume 203, Number 4, pp. 605-14

Published in 2013 Nov 25

(Abstract) Epithelial cells characteristically have noncentrosomal microtubules that are arranged in the apicobasal direction. In this paper, we examined cell sheets formed by an epithelial (Eph4) cell line by structure illumination microscopy and found a previously not clearly described planar apical network of noncentrosomal microtubules (MTs) in which the sides of the MT bundles were associated with tight junctions (TJs). In a gel overlay assay with taxol-stabilized MTs, cingulin showed strong binding to MTs, and a domain analysis showed that this binding occurred through cingulin's N-terminal region. The association of planar apical MTs with TJs was compromised by cingulin knockdown (KD) or the expression of dephosphomimetic mutants of cingulin at its adenosine monophosphate-activated protein kinase (AMPK) target sites, whereas phosphorylation at these sites facilitated cingulin-tubulin binding. In addition, although wild-type colonies formed spheres in 3D culture, the cingulin KD cells had anisotropic shapes. These findings collectively suggest that the regulated cingulin-MT association has a specific role in TJ-related epithelial morphogenesis that is sensitive to metabolic homeostasis-related AMPK activity.
(MeSH Terms)

Contact
Sachiko Tsukita , Osaka University , Graduate School of Frontier Biosciences and Medicine , Laboratory of Biological Science
Contributors
Tomoki Yano, Takeshi Matsui, Atsushi Tamura, Masami Uji, Sachiko Tsukita


Dataset List of 59-Yano-MicrotubuleImmuno

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 1378
Dataset Kind Image data
Dataset Size 1.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data