Summary of 52-Suzuki-FrogTailDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
16.9 MB ( Image datasets: 16.9 MB, Quantitative data datasets: 0 bytes )

Organism(s)
X. laevis
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
chromatin remodeling
Cellular Component (CC)
nucleus
Biological Imaging Method
-
XYZ Scale
XY: 5.7 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
TimeLapse
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
Leica MZ10F
Detector model
Nikon Digital sight Ds-Vi1
Objective model
-
Filter set
Leica GFP LP

Related paper(s)

Miyuki Suzuki, Chiyo Takagi, Shinichirou Miura, Yuto Sakane, Makoto Suzuki, Tetsushi Sakuma, Naoaki Sakamoto, Tetsuya Endo, Yasuhiro Kamei, Yuko Sato, Hiroshi Kimura, Takashi Yamamoto, Naoto Ueno, Ken-ichi T Suzuki (2016) In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration., Genes to cells : devoted to molecular & cellular mechanisms, Volume 21, Number 4, pp. 358-69

Published in 2016 Apr (Electronic publication in Feb. 24, 2016, midnight )

(Abstract) Xenopus laevis tadpoles can completely regenerate their appendages, such as tail and limbs, and therefore provide a unique model to decipher the molecular mechanisms of organ regeneration in vertebrates. Epigenetic modifications are likely to be involved in this remarkable regeneration capacity, but they remain largely unknown. To examine the involvement of histone modification during organ regeneration, we generated transgenic X. laevis ubiquitously expressing a fluorescent modification-specific intracellular antibody (Mintbody) that is able to track histone H3 lysine 9 acetylation (H3K9ac) in vivo through nuclear enhanced green fluorescent protein (EGFP) fluorescence. In embryos ubiquitously expressing H3K9ac-Mintbody, robust fluorescence was observed in the nuclei of somites. Interestingly, H3K9ac-Mintbody signals predominantly accumulated in nuclei of regenerating notochord at 24 h postamputation following activation of reactive oxygen species (ROS). Moreover, apocynin (APO), an inhibitor of ROS production, attenuated H3K9ac-Mintbody signals in regenerating notochord. Our results suggest that ROS production is involved in acetylation of H3K9 in regenerating notochord at the onset of tail regeneration. We also show this transgenic Xenopus to be a useful tool to investigate epigenetic modification, not only in organogenesis but also in organ regeneration.
(MeSH Terms)

Contact
Ken-ichi T. Suzuki , Hiroshima University , Department of Mathematical and Life Sciences , Molecular Genetics Laboratory
Contributors
Miyuki Suzuki, Chiyo Takagi, Shinichirou Miura, Yuto Sakane, Makoto Suzuki, Tetsushi Sakuma, Naoaki Sakamoto, Tetsuya Endo, Yasuhiro Kamei, Yuko Sato, Hiroshi Kimura, Takashi Yamamoto, Naoto Ueno, Ken-ichi T. Suzuki


Dataset List of 52-Suzuki-FrogTailDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 1347
Datast ID Fig3_amputation
Dataset Kind Image data
Dataset Size 16.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data