SSBD:database

Summary of 389-Watabe-PGE2discharge

SSBD:database
389-Watabe-PGE2discharge
SSBD:database URL
https://ssbd.riken.jp/database/389-Watabe-PGE2discharge/
SSBD:repository
ssbd-repos-000389
SSBD:repository URL
https://ssbd.riken.jp/repository/389/
Title
Visual quantification of prostaglandin E2 discharge from a single cell
Description
Calcium transients drive cells to discharge prostaglandin E2 (PGE2). The authors visualized PGE2-induced protein kinase A (PKA) activation and quantitated PGE2 secreted from a single cell by combining fluorescence microscopy and a simulation model. For this purpose, they first prepared PGE2-producer cells that express either an optogenetic or a chemogenetic calcium channel stimulator: OptoSTIM1 (CRY2clust) or Gq-DREADD, respectively. Second, they prepared reporter cells expressing the Gs-coupled PGE2 reporter EP2 and the PKA biosensor Booster-PKA, which is based on the principle of Forster resonance FRET. Upon the stimulation-induced triggering of calcium transients, a single producer cell discharges PGE2 to stimulate PKA in the surrounding reporter cells. Due to the flow of the medium, the PKA-activated area exhibited a comet-like smear when HeLa cells were used. In contrast, radial PKA activation was observed when confluent MDCK cells were used, indicating that PGE2 diffusion was restricted to the basolateral space. By fitting the radius of the PKA-activated area to a simulation model based on simple diffusion, the authors estimated that a single HeLa cell secretes 0.25 fmol PGE2 upon a single calcium transient to activate PKA in more than 1000 neighboring cells. This model also predicts that the PGE2 discharge rate is comparable to the diffusion rate. Thus, their method quantitatively envisions that a single calcium transient affects more than 1000 neighboring cells via PGE2.
Release date
2026-01-21
Updated date
-
License
CC BY 4.0
Kind
Image data based on Experiment
Number of Datasets
146 ( Image datasets: 146, Quantitative data datasets: 0 )
Size of Datasets
77.0 GB ( Image datasets: 77.0 GB, Quantitative data datasets: 0 bytes )
Organism(s)
Homo sapiens, Canis lupus familiaris
Cell lines(s)
HeLa cell, MDCK cell
Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
signal transduction, cell communication
Cellular Component (CC)
cytoplasm
Biological Imaging Method
time lapse microscopy, FRET, fluorescence microscopy
T scale
NA, 1 minute
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Related paper(s)

Tetsuya Watabe, Shinya Yamahira, Michiyuki Matsuda, Kenta Terai (2023) Visual quantification of prostaglandin E(2) discharge from a single cell., Cell structure and function

Published in 2023 Oct 7 (Electronic publication in Oct. 7, 2023, midnight )

(Abstract) Calcium transients drive cells to discharge prostaglandin E(2) (PGE(2)). We visualized PGE(2)-induced protein kinase A (PKA) activation and quantitated PGE(2) secreted from a single cell by combining fluorescence microscopy and a simulation model. For this purpose, we first prepared PGE(2)-producer cells that express either an optogenetic or a chemogenetic calcium channel stimulator: OptoSTIM1 or Gq-DREADD, respectively. Second, we prepared reporter cells expressing the Gs-coupled PGE(2) reporter EP2 and the PKA biosensor Booster-PKA, which is based on the principle of Forster resonance energy transfer. Upon the stimulation-induced triggering of calcium transients, a single producer cell discharges PGE(2) to stimulate PKA in the surrounding reporter cells. Due to the flow of the medium, the PKA-activated area exhibited a comet-like smear when HeLa cells were used. In contrast, radial PKA activation was observed when confluent MDCK cells were used, indicating that PGE(2) diffusion was restricted to the basolateral space. By fitting the radius of the PKA-activated area to a simulation model based on simple diffusion, we estimated that a single HeLa cell secretes 0.25 fmol PGE(2) upon a single calcium transient to activate PKA in more than 1000 neighboring cells. This model also predicts that the PGE(2) discharge rate is comparable to the diffusion rate. Thus, our method quantitatively envisions that a single calcium transient affects more than 1000 neighboring cells via PGE(2).Keywords: prostaglandin E(2), imaging, intercellular communication, biosensor, quantification.
Contact
Kenta Terai , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors
Tetsuya Watabe, Shinya Yamahira

Dataset List of 389-Watabe-PGE2discharge

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 13662
Datast ID Fig1DE_HeLa_PKA
Dataset Kind Image data
Dataset Size 5.1 MB
4D view
SSBD:OMERO
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# 13663
Datast ID Fig1F_MDCK_PKA
Dataset Kind Image data
Dataset Size 8.3 MB
4D view
SSBD:OMERO
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# 13664
Datast ID Fig1G_HeLa_Comet
Dataset Kind Image data
Dataset Size 969.6 MB
4D view
SSBD:OMERO
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# 13665
Datast ID Fig1G_MDCK_NotComet
Dataset Kind Image data
Dataset Size 969.6 MB
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SSBD:OMERO
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# 13666
Datast ID Fig2A_0nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13667
Datast ID Fig2A_0.1nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13668
Datast ID Fig2A_0.3nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13669
Datast ID Fig2A_1nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13670
Datast ID Fig2A_3nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13671
Datast ID Fig2A_10nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13672
Datast ID Fig2A_100nM
Dataset Kind Image data
Dataset Size 6.7 MB
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SSBD:OMERO
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# 13673
Datast ID Fig2DE_HeLa_PKA_1
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13674
Datast ID Fig2DE_HeLa_PKA_2
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13675
Datast ID Fig2DE_HeLa_PKA_3
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13676
Datast ID Fig2DE_HeLa_PKA_4
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13677
Datast ID Fig2DE_HeLa_PKA_5
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13678
Datast ID Fig2DE_HeLa_PKA_6
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13679
Datast ID Fig2DE_HeLa_PKA_7
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13680
Datast ID Fig2DE_HeLa_PKA_8
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13681
Datast ID Fig2G_MDCK_PKA_1
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13682
Datast ID Fig2G_MDCK_PKA_2
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13683
Datast ID Fig2G_MDCK_PKA_3
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13684
Datast ID Fig2G_MDCK_PKA_4
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13685
Datast ID Fig2G_MDCK_PKA_5
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13686
Datast ID Fig2G_MDCK_PKA_6
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13687
Datast ID Fig2G_MDCK_PKA_7
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13688
Datast ID Fig2G_MDCK_PKA_8
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13689
Datast ID Fig2G_MDCK_PKA_9
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13690
Datast ID Fig2G_MDCK_PKA_10
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13691
Datast ID Fig2G_MDCK_PKA_11
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13692
Datast ID Fig2G_MDCK_PKA_12
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13693
Datast ID Fig2G_MDCK_PKA_13
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13694
Datast ID Fig2G_MDCK_PKA_14
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13695
Datast ID Fig2G_MDCK_PKA_15
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13696
Datast ID Fig2G_MDCK_PKA_16
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13697
Datast ID Fig2G_MDCK_PKA_17
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13698
Datast ID Fig2G_MDCK_PKA_18
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13699
Datast ID Fig2G_MDCK_PKA_19
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13700
Datast ID Fig2G_MDCK_PKA_20
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
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# 13701
Datast ID Fig2G_MDCK_PKA_21
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13702
Datast ID Fig2G_MDCK_PKA_22
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13703
Datast ID Fig2G_MDCK_PKA_23
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13704
Datast ID Fig2G_MDCK_PKA_24
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13705
Datast ID Fig2G_MDCK_PKA_25
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13706
Datast ID Fig2G_MDCK_PKA_26
Dataset Kind Image data
Dataset Size 549.9 MB
4D view
SSBD:OMERO
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# 13707
Datast ID Fig2G_MDCK_PKA_27
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13708
Datast ID Fig2G_MDCK_PKA_28
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13709
Datast ID Fig2G_MDCK_PKA_29
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13710
Datast ID Fig2G_MDCK_PKA_30
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13711
Datast ID Fig2G_MDCK_PKA_31
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13712
Datast ID Fig2G_MDCK_PKA_32
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13713
Datast ID Fig2G_MDCK_PKA_33
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13714
Datast ID Fig2G_MDCK_PKA_34
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13715
Datast ID Fig2G_MDCK_PKA_35
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13716
Datast ID Fig2G_MDCK_PKA_36
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13717
Datast ID Fig2G_MDCK_PKA_37
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13718
Datast ID Fig2G_MDCK_PKA_38
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13719
Datast ID Fig2G_MDCK_PKA_39
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13720
Datast ID Fig2G_MDCK_PKA_40
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13721
Datast ID Fig2G_MDCK_PKA_41
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13722
Datast ID Fig2G_MDCK_PKA_42
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13723
Datast ID Fig2G_MDCK_PKA_43
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13724
Datast ID Fig2G_MDCK_PKA_44
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13725
Datast ID Fig2G_MDCK_PKA_45
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13726
Datast ID Fig2G_MDCK_PKA_46
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13727
Datast ID Fig2G_MDCK_PKA_47
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13728
Datast ID Fig2G_MDCK_PKA_48
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13729
Datast ID Fig2G_MDCK_PKA_49
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13730
Datast ID Fig2G_MDCK_PKA_50
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13731
Datast ID Fig2G_MDCK_PKA_51
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13732
Datast ID Fig2G_MDCK_PKA_52
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13733
Datast ID Fig2G_MDCK_PKA_53
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13734
Datast ID Fig2G_MDCK_PKA_54
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13735
Datast ID Fig2G_MDCK_PKA_55
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13736
Datast ID Fig2G_MDCK_PKA_56
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13737
Datast ID Fig2G_MDCK_PKA_57
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13738
Datast ID Fig2G_MDCK_PKA_58
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13739
Datast ID Fig2G_MDCK_PKA_59
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13740
Datast ID Fig2G_MDCK_PKA_60
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13741
Datast ID Fig2G_MDCK_PKA_61
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13742
Datast ID Fig2G_MDCK_PKA_62
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13743
Datast ID Fig2G_MDCK_PKA_63
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13744
Datast ID Fig2G_MDCK_PKA_64
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13745
Datast ID Fig2G_MDCK_PKA_65
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13746
Datast ID Fig2G_MDCK_PKA_66
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13747
Datast ID Fig2G_MDCK_PKA_67
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13748
Datast ID Fig2G_MDCK_PKA_68
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13749
Datast ID Fig2G_MDCK_PKA_69
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13750
Datast ID Fig2G_MDCK_PKA_70
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13751
Datast ID Fig2G_MDCK_PKA_71
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13752
Datast ID Fig2G_MDCK_PKA_72
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13753
Datast ID Fig2G_MDCK_PKA_73
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13754
Datast ID Fig2G_MDCK_PKA_74
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13755
Datast ID Fig2G_MDCK_PKA_75
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13756
Datast ID Fig2G_MDCK_PKA_76
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13757
Datast ID Fig2G_MDCK_PKA_77
Dataset Kind Image data
Dataset Size 549.9 MB
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# 13758
Datast ID Fig2G_MDCK_PKA_78
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13759
Datast ID Fig2G_MDCK_PKA_79
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13760
Datast ID Fig2G_MDCK_PKA_80
Dataset Kind Image data
Dataset Size 549.9 MB
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SSBD:OMERO
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# 13761
Datast ID Fig3AB_PKA_ctrl_1
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13762
Datast ID Fig3AB_PKA_ctrl_2
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13763
Datast ID Fig3AB_PKA_ctrl_3
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13764
Datast ID Fig3AB_PKA_ctrl_4
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13765
Datast ID Fig3AB_PKA_ctrl_5
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13766
Datast ID Fig3AB_PKA_ctrl_6
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13767
Datast ID Fig3AB_PKA_ctrl_7
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13768
Datast ID Fig3AB_PKA_ctrl_8
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13769
Datast ID Fig3AB_PKA_ctrl_9
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13770
Datast ID Fig3AB_PKA_ctrl_10
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13771
Datast ID Fig3AB_PKA_ctrl_11
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13772
Datast ID Fig3AB_PKA_ctrl_12
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13773
Datast ID Fig3AB_PKA_ctrl_13
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13774
Datast ID Fig3AB_PKA_ctrl_14
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13775
Datast ID Fig3AB_PKA_EGF_1
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13776
Datast ID Fig3AB_PKA_EGF_2
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13777
Datast ID Fig3AB_PKA_EGF_3
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13778
Datast ID Fig3AB_PKA_EGF_4
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13779
Datast ID Fig3AB_PKA_EGF_5
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13780
Datast ID Fig3AB_PKA_EGF_6
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13781
Datast ID Fig3AB_PKA_EGF_7
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13782
Datast ID Fig3AB_PKA_EGF_8
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13783
Datast ID Fig3AB_PKA_EGF_9
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13784
Datast ID Fig3AB_PKA_EGF_10
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13785
Datast ID Fig3AB_PKA_EGF_11
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13786
Datast ID Fig3AB_PKA_EGF_12
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13787
Datast ID Fig3AB_PKA_EGF_13
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13788
Datast ID Fig3AB_PKA_EGF_14
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13789
Datast ID Fig3AB_PKA_MEKi_1
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13790
Datast ID Fig3AB_PKA_MEKi_2
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13791
Datast ID Fig3AB_PKA_MEKi_3
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13792
Datast ID Fig3AB_PKA_MEKi_4
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13793
Datast ID Fig3AB_PKA_MEKi_5
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13794
Datast ID Fig3AB_PKA_MEKi_6
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13795
Datast ID Fig3AB_PKA_MEKi_7
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13796
Datast ID Fig3AB_PKA_MEKi_8
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13797
Datast ID Fig3AB_PKA_MEKi_9
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13798
Datast ID Fig3AB_PKA_MEKi_10
Dataset Kind Image data
Dataset Size 553.6 MB
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# 13799
Datast ID Fig3AB_PKA_MEKi_11
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13800
Datast ID Fig3AB_PKA_MEKi_12
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13801
Datast ID Fig3AB_PKA_MEKi_13
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13802
Datast ID Fig3AB_PKA_MEKi_14
Dataset Kind Image data
Dataset Size 553.6 MB
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SSBD:OMERO
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# 13803
Datast ID FigS1_HeLa_ctrl
Dataset Kind Image data
Dataset Size 213.1 MB
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SSBD:OMERO
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# 13804
Datast ID FigS1_HeLa_inhibit
Dataset Kind Image data
Dataset Size 213.1 MB
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SSBD:OMERO
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# 13805
Datast ID FigS1_MDCK_ctrl
Dataset Kind Image data
Dataset Size 159.9 MB
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SSBD:OMERO
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# 13806
Datast ID FigS1_MDCK_inhibit
Dataset Kind Image data
Dataset Size 159.9 MB
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SSBD:OMERO
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# 13807
Datast ID FigS2_EcadKO_PKA
Dataset Kind Image data
Dataset Size 4.4 GB
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SSBD:OMERO
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