Detail of Fig2A_10nM


Project
SSBD:Repository
Title
FRET images of Booster-PKA in reporter COX-DKO-HeLa cells treated with 10 nM PGE2
Description
FRET images of Booster-PKA in reporter COX-DKO-HeLa cells (COX-1 and COX-2 double knockout HeLa cells) treated with 10 nM PGE2 (prostaglandin E2) for 20 to 30 min. The data of 3 independent experiments are reposited.
Release, Updated
2026-01-21
License
CC BY 4.0
Kind
Image data
File Formats
.nd2
Data size
6.7 MB

Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
-
Cell Line
HeLa cell

Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell communication , signal transduction
Cellular Component (CC)
cytoplasm
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
FRET ( Fbbi:00000367 )
X scale
2.7 micrometer
Y scale
2.7 micrometer
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Watabe T, Yamahira S, Matsuda M, Terai K. Visual quantification of prostaglandin E2 discharge from a single cell. Cell Struct Funct . 2023 Dec 7;48(2):241-249.
Related paper(s)

Contact
Kenta Terai , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors
Tetsuya Watabe, Shinya Yamahira

OMERO Dataset
OMERO Project
Source