Summary of 31-Matsuda-SigDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
3 ( Image datasets: 3, Quantitative data datasets: 0 )
Size of Datasets
252.0 MB ( Image datasets: 252.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
C. l. familiaris, C. griseus
Strain(s)
MDCK
Gene symbol(s)
NA
Protein name(s)
Delta
Protein tag(s)
GFP

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.8 micrometer/pixel, Z: NA, XY: 1.0 micrometer/pixel, Z: NA
T scale
0.333 hour for each time interval, 0.25 hour for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Mitsuhiro Matsuda, Makito Koga, Eisuke Nishida, Miki Ebisuya (2012) Synthetic signal propagation through direct cell-cell interaction., Science signaling, Volume 5, Number 220, pp. ra31

Published in 2012 Apr 17 (Electronic publication in April 17, 2012, midnight )

(Abstract) Contact-dependent cell communication has the potential to generate elaborate cell patterns, and this occurs in vivo. We used the Delta-Notch signaling system, consisting of the ligand Delta and the receptor Notch, to construct a positive feedback loop between adjacent cells to generate a propagating signal in cultured cells. To amplify the responses of Notch to Delta, we created a cell-cell positive feedback loop using an engineered transcriptional cascade and a Notch positive regulator, Lunatic fringe. We used mathematical modeling to determine the appropriate amount of amplification to enable the induction of Delta to propagate from one cell to its neighboring cells, which generated bistability within the local cell populations and resulted in discrete groups of cells that were either positive or negative for Delta. These results demonstrate the sufficiency of the cell-cell positive feedback loop to generate signal propagation and cell population-level bistability. This study represents a step in engineering more elaborate cell patterns in mammalian cells.
(MeSH Terms)

Contact
Miki Ebisuya , RIKEN , Quantitative Biology Center , Laboratory for Reconstitutive Developmental Biology
Contributors
Mitsuhiro Matsuda, Makito Koga, Eisuke Nishida, Miki Ebisuya


Dataset List of 31-Matsuda-SigDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 971
Datast ID Fig5A_WT
Dataset Kind Image data
Dataset Size 72.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 972
Datast ID Fig5A_Trigger
Dataset Kind Image data
Dataset Size 72.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 973
Datast ID Fig5C
Dataset Kind Image data
Dataset Size 106.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data