Summary of 30-Inomata-CellDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
Number of Datasets
5 ( Image datasets: 5, Quantitative data datasets: 0 )
Size of Datasets
1.2 GB ( Image datasets: 1.2 GB, Quantitative data datasets: 0 bytes )

Organism(s)
X. laevis
Gene symbol(s)
NA
Protein name(s)
Chordin, Noggin, Sizzled, TM
Protein tag(s)
mEGFP

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
NA (protein diffusion)
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.267 micrometer/pixel, Z: 0 micrometer/frame
T scale
0.97 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM710
Detector model
-
Objective model
Carl Zeiss Plan-Apochromat 20x/0.8
Filter set
-

Related paper(s)

Hidehiko Inomata, Tatsuo Shibata, Tomoko Haraguchi, Yoshiki Sasai (2013) Scaling of dorsal-ventral patterning by embryo size-dependent degradation of Spemann's organizer signals., Cell, Volume 153, Number 6, pp. 1296-311

Published in 2013 Jun 6

(Abstract) Spemann's organizer plays a key role in dorsal-ventral (DV) patterning in the amphibian embryo by secreting diffusible proteins such as Chordin, an antagonist to ventralizing bone morphogenetic proteins (BMPs). The DV patterning is so robust that an amphibian embryo with its ventral half surgically removed can develop into a smaller but proportionally patterned larva. Here, we show that this robust patterning depends on facilitated Chordin degradation and requires the expression of the Chordin-proteinase inhibitor Sizzled on the opposite side. Sizzled, which is stable and diffuses widely along the DV axis, stabilizes Chordin and expands its distribution in the ventral direction. This expanded Chordin distribution, in turn, limits BMP-dependent Sizzled production, forming an axis-wide feedback loop for shaping Chordin's activity. Using bisection assays, we demonstrate that Chordin degradation is dynamically controlled by embryo-size-coupled Sizzled accumulation. We propose a scaling model that enables the DV pattern to adjust proportionally to embryonic axis size.
(MeSH Terms)

Contact
Hidehiko Inomata , RIKEN , Center for Developmental Biology , Laboratory for Physical Biology
Contributors
Hidehiko Inomata, Tatsuo Shibata, Tomoko Haraguchi, Yoshiki Sasai


Dataset List of 30-Inomata-CellDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 966
Datast ID MovieS3-EG-Chd
Dataset Kind Image data
Dataset Size 290.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 967
Datast ID MovieS4-EG-Nog
Dataset Kind Image data
Dataset Size 232.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 968
Datast ID MovieS5-EG-Szl
Dataset Kind Image data
Dataset Size 270.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 969
Datast ID MovieS2-EG-TM
Dataset Kind Image data
Dataset Size 141.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 970
Datast ID MovieS1-EG
Dataset Kind Image data
Dataset Size 292.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data