SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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289-Fujimoto-MitralCell

https://ssbd.riken.jp/database/289-Fujimoto-MitralCell/

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ssbd-repos-000289

https://ssbd.riken.jp/repository/289/

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Image data showing activity-dependent dendrite remodeling in the developing mouse olfactory bulb and barrel cortex

In developing brains, activity-dependent remodeling facilitates the formation of precise neuronal connectivity. Synaptic competition is known to facilitate synapse elimination; however, it has remained unknown how different synapses compete with one another within a post-synaptic cell. Here, the authors investigate how a mitral cell in the mouse olfactory bulb prunes all but one primary dendrite during the developmental remodeling process. They find that spontaneous activity generated within the olfactory bulb is essential. They show that strong glutamatergic inputs to one dendrite trigger branch-specific changes in RhoA activity to facilitate the pruning of the remaining dendrites: NMDAR-dependent local signals suppress RhoA to protect it from pruning; however, the subsequent neuronal depolarization induces neuron-wide activation of RhoA to prune non-protected dendrites. NMDAR-RhoA signals are also essential for the synaptic competition in the mouse barrel cortex. These results demonstrate a general principle whereby activity-dependent lateral inhibition across synapses establishes a discrete receptive field of a neuron.

2026-08-07

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CC BY 4.0

Image data based on Experiment

461 ( Image datasets: 461, Quantitative data datasets: 0 )

751.2 GB ( Image datasets: 751.2 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

C57BL/6N, ICR

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neuron development, neuron development neuron remodeling, postsynaptic signal transduction, calcium ion transport, negative regulation of neuron remodeling, calcium ion transport response to odorant

dendrite glutamatergic synapse, cell body dendrite, cytoplasm presynapse, dendrite, axon

two-photon laser scanning microscopy, confocal microscopy

0.564 seconds 0.842 seconds 0.429 seconds 0.429 seconds, 0.151 seconds, 0.032 seconds, 0.429 seconds 0.429 seconds 0.429 seconds 0.429 seconds, 10 seconds, 0.564 seconds 0.564 seconds 0.564 seconds, 0.429 seconds 0.429 seconds 0.429 seconds, 0.429 seconds 0.429 seconds, 0.375 seconds 0.397 seconds, 0.429 seconds 0.429 seconds 0.429 seconds 0.429 seconds 0.429 seconds 0.564 seconds, 0.564 seconds, 0.4 seconds, 0.429 seconds, 5 seconds, NA, 0.035 seconds, 0.43 seconds

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Satoshi Fujimoto, Marcus N Leiwe, Shuhei Aihara, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai (2023) Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice., Developmental cell

Published in June 7, 2023 (Electronic publication in May 30, 2023, midnight )

• doi: 10.1016/j.devcel.2023.05.004
• pmid: 37290446
  
• url: https://www.sciencedirect.com/science/article/pii/S153458072300237X
  

(Abstract) In developing brains, activity-dependent remodeling facilitates the formation of precise neuronal connectivity. Synaptic competition is known to facilitate synapse elimination; however, it has remained unknown how different synapses compete with one another within a post-synaptic cell. Here, we investigate how a mitral cell in the mouse olfactory bulb prunes all but one primary dendrite during the developmental remodeling process. We find that spontaneous activity generated within the olfactory bulb is essential. We show that strong glutamatergic inputs to one dendrite trigger branch-specific changes in RhoA activity to facilitate the pruning of the remaining dendrites: NMDAR-dependent local signals suppress RhoA to protect it from pruning; however, the subsequent neuronal depolarization induces neuron-wide activation of RhoA to prune non-protected dendrites. NMDAR-RhoA signals are also essential for the synaptic competition in the mouse barrel cortex. Our results demonstrate a general principle whereby activity-dependent lateral inhibition across synapses establishes a discrete receptive field of a neuron.

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Takeshi Imai, , Kyushu University, , Graduate School of Medical Sciences, , Graduate School of Medical Sciences,

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