SSBD:database

Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki, Kenta Terai, Sachiko Komazawa-Sakon, Takao Seki, Yoshifumi Yamaguchi, Tetuo Mikami, Kimi Araki, Masaki Ohmuraya, Michiyuki Matsuda, Hiroyasu Nakano (2022) Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis., Communications biology, Volume 5, Number 1, pp. 1331

Published in 2022 Dec 5 (Electronic publication in Dec. 5, 2022, midnight )

• doi: 10.1038/s42003-022-04300-0
• pmid: 36471162
  

(Abstract) Necroptosis is a regulated form of cell death involved in various pathological conditions, including ischemic reperfusion injuries, virus infections, and drug-induced tissue injuries. However, it is not fully understood when and where necroptosis occurs in vivo. We previously generated a Forster resonance energy transfer (FRET) biosensor, termed SMART (the sensor for MLKL activation by RIPK3 based on FRET), which monitors conformational changes of MLKL along with progression of necroptosis in human and murine cell lines in vitro. Here, we generate transgenic (Tg) mice that express the SMART biosensor in various tissues. The FRET ratio is increased in necroptosis, but not apoptosis or pyroptosis, in primary cells. Moreover, the FRET signals are elevated in renal tubular cells of cisplatin-treated SMART Tg mice compared to untreated SMART Tg mice. Together, SMART Tg mice may provide a valuable tool for monitoring necroptosis in different types of cells in vitro and in vivo.

• Humans
• Mice
• Animals
• Necroptosis[major]
• Fluorescence Resonance Energy Transfer
• Mice, Transgenic
• Protein Kinases/metabolism
• Biosensing Techniques[major]