Summary of 272-Yoshida-EGFRSignalingDyn

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SSBD:repository URL

Title
Time-lapse or z-stack images of EGFR signaling dynamics in epithelial homeostasis of Drosophila embyos.
Description
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Relase date
2023-08-29
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
28 ( Image datasets: 28, Quantitative data datasets: 0 )
Size of Datasets
73.6 GB ( Image datasets: 73.6 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Drosophila melanogaster

Datatype
-
Molecular Function (MF)
MAP kinase activity, epidermal growth factor receptor activity
Biological Process (BP)
tissue homeostasis, execution phase of apoptosis, wound healing, epithelial cell morphogenesis, epithelial structure maintenance
Cellular Component (CC)
-
Biological Imaging Method
time lapse microscopy, confocal microscopy, laser scanning confocal microscope
T scale
5 minutes of time interval, 3 minutes of time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
-
Filter set
-

Related paper(s)

Kentaro Yoshida, Shigeo Hayashi (2023) Epidermal growth factor receptor signaling protects epithelia from morphogenetic instability and tissue damage in Drosophila., Development (Cambridge, England), Volume 150, Number 5

Published in 2023 Mar 1 (Electronic publication in March 9, 2023, midnight )

(Abstract) Dying cells in the epithelia communicate with neighboring cells to initiate coordinated cell removal to maintain epithelial integrity. Naturally occurring apoptotic cells are mostly extruded basally and engulfed by macrophages. Here, we have investigated the role of Epidermal growth factor (EGF) receptor (EGFR) signaling in the maintenance of epithelial homeostasis. In Drosophila embryos, epithelial tissues undergoing groove formation preferentially enhanced extracellular signal-regulated kinase (ERK) signaling. In EGFR mutant embryos at stage 11, sporadic apical cell extrusion in the head initiates a cascade of apical extrusions of apoptotic and non-apoptotic cells that sweeps the entire ventral body wall. Here, we show that this process is apoptosis dependent, and clustered apoptosis, groove formation, and wounding sensitize EGFR mutant epithelia to initiate massive tissue disintegration. We further show that tissue detachment from the vitelline membrane, which frequently occurs during morphogenetic processes, is a key trigger for the EGFR mutant phenotype. These findings indicate that, in addition to cell survival, EGFR plays a role in maintaining epithelial integrity, which is essential for protecting tissues from transient instability caused by morphogenetic movement and damage.
(MeSH Terms)

Contact
Shigeo Hayashi , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Morphogenetic Signalin
Contributors


Dataset List of 272-Yoshida-EGFRSignalingDyn

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Dataset ID
Kind
Size
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# 10812
Dataset Kind Image data
Dataset Size 5.0 GB
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SSBD:OMERO
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# 10813
Dataset Kind Image data
Dataset Size 3.3 GB
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SSBD:OMERO
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# 10814
Dataset Kind Image data
Dataset Size 1.1 GB
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SSBD:OMERO
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# 10815
Dataset Kind Image data
Dataset Size 1.2 GB
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SSBD:OMERO
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# 10816
Dataset Kind Image data
Dataset Size 3.2 GB
4D view
SSBD:OMERO
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# 10817
Dataset Kind Image data
Dataset Size 2.6 GB
4D view
SSBD:OMERO
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# 10818
Dataset Kind Image data
Dataset Size 372.0 MB
4D view
SSBD:OMERO
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# 10819
Dataset Kind Image data
Dataset Size 372.0 MB
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SSBD:OMERO
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# 10820
Dataset Kind Image data
Dataset Size 372.0 MB
4D view
SSBD:OMERO
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# 10821
Dataset Kind Image data
Dataset Size 372.0 MB
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SSBD:OMERO
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# 10822
Dataset Kind Image data
Dataset Size 372.0 MB
4D view
SSBD:OMERO
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# 10823
Dataset Kind Image data
Dataset Size 372.0 MB
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SSBD:OMERO
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# 10824
Dataset Kind Image data
Dataset Size 9.3 GB
4D view
SSBD:OMERO
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# 10825
Dataset Kind Image data
Dataset Size 186.5 MB
4D view
SSBD:OMERO
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# 10826
Dataset Kind Image data
Dataset Size 186.5 MB
4D view
SSBD:OMERO
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# 10827
Dataset Kind Image data
Dataset Size 372.0 MB
4D view
SSBD:OMERO
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# 10828
Dataset Kind Image data
Dataset Size 372.0 MB
4D view
SSBD:OMERO
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# 10829
Dataset Kind Image data
Dataset Size 11.1 GB
4D view
SSBD:OMERO
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# 10830
Dataset Kind Image data
Dataset Size 8.6 GB
4D view
SSBD:OMERO
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# 10831
Dataset Kind Image data
Dataset Size 7.2 GB
4D view
SSBD:OMERO
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# 10832
Dataset Kind Image data
Dataset Size 5.5 GB
4D view
SSBD:OMERO
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# 10833
Dataset Kind Image data
Dataset Size 4.6 GB
4D view
SSBD:OMERO
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# 10834
Dataset Kind Image data
Dataset Size 113.1 MB
4D view
SSBD:OMERO
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# 10835
Dataset Kind Image data
Dataset Size 904.7 MB
4D view
SSBD:OMERO
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# 10836
Dataset Kind Image data
Dataset Size 95.4 MB
4D view
SSBD:OMERO
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# 10837
Dataset Kind Image data
Dataset Size 1.6 GB
4D view
SSBD:OMERO
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# 10838
Dataset Kind Image data
Dataset Size 2.7 GB
4D view
SSBD:OMERO
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# 10839
Dataset Kind Image data
Dataset Size 2.2 GB
4D view
SSBD:OMERO
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