Detail of Fig3G_Par5-GFP_egfr
Project
SSBD:Repository
Title
Time-lapse images of the head region of the EGFR mutant showing an extensive collapse in the ocular segment of the head.
Description
Time-lapse images of the head region of the EGFR mutant showing an extensive collapse in the ocular segment of the head.
Release, Updated
2023-08-29
License
CC BY
Kind
Image data
File Formats
.oib
Data size
9.3 GB
Datatype
-
Molecular Function (MF)
epidermal growth factor receptor activity
(
GO:0005006
)
Biological Process (BP)
epithelial cell morphogenesis
(
GO:0003382
)
tissue homeostasis
(
GO:0001894
)
epithelial structure maintenance
(
GO:0010669
)
execution phase of apoptosis
(
GO:0097194
)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.187 micrometer/pixel
Y scale
0.187 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
3 minutes of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Yoshida K, et. al. Development. 2023 Mar 1;150(5):dev201231.
Related paper(s)
Kentaro Yoshida, Shigeo Hayashi (2023) Epidermal growth factor receptor signaling protects epithelia from morphogenetic instability and tissue damage in Drosophila., Development (Cambridge, England), Volume 150, Number 5
Published in 2023 Mar 1 (Electronic publication in March 9, 2023, midnight )
- doi: 10.1242/dev.201231
-
pmid: 36897356
(Abstract) Dying cells in the epithelia communicate with neighboring cells to initiate coordinated cell removal to maintain epithelial integrity. Naturally occurring apoptotic cells are mostly extruded basally and engulfed by macrophages. Here, we have investigated the role of Epidermal growth factor (EGF) receptor (EGFR) signaling in the maintenance of epithelial homeostasis. In Drosophila embryos, epithelial tissues undergoing groove formation preferentially enhanced extracellular signal-regulated kinase (ERK) signaling. In EGFR mutant embryos at stage 11, sporadic apical cell extrusion in the head initiates a cascade of apical extrusions of apoptotic and non-apoptotic cells that sweeps the entire ventral body wall. Here, we show that this process is apoptosis dependent, and clustered apoptosis, groove formation, and wounding sensitize EGFR mutant epithelia to initiate massive tissue disintegration. We further show that tissue detachment from the vitelline membrane, which frequently occurs during morphogenetic processes, is a key trigger for the EGFR mutant phenotype. These findings indicate that, in addition to cell survival, EGFR plays a role in maintaining epithelial integrity, which is essential for protecting tissues from transient instability caused by morphogenetic movement and damage.(MeSH Terms)
Contact
Shigeo Hayashi
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Morphogenetic Signalin
Contributors
OMERO Project
Source