Summary of 268-Oishi-VesselClassification

SSBD:database
SSBD:database URL
Title
Confocal immunohistochemistry images and BDML file for quantitative information of SMA/laminin α2 mouse vessel-type classification for machine learning.
Description
-
Relase date
2023-09-01
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
21 ( Image datasets: 21, Quantitative data datasets: 0 )
Size of Datasets
8.1 GB ( Image datasets: 8.1 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Strain(s)
STOCK
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
laminin binding
Biological Process (BP)
arteriole smooth muscle contraction
Cellular Component (CC)
laminin-2 complex
Biological Imaging Method
confocal microscopy
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Mitsuhiro Oishi, Stefan Passlick, Yoshihiko Yamazaki, Miyuki Unekawa, Ruka Adachi, Mayumi Yamada, Itaru Imayoshi, Yoshifumi Abe, Christian Steinhauser, Kenji F Tanaka (2022) Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter., Glia

Published in 2022 Sep 27 (Electronic publication in Sept. 27, 2022, midnight )

(Abstract) Nerve/glial antigen 2 (NG2) is a protein marker of NG2 glia and mural cells, and NG2 promoter activity is utilized to target these cells. However, the NG2 promoter cannot target NG2 glia and mural cells separately. This has been an obstacle for NG2 glia-specific manipulation. Here, we developed transgenic mice in which either cell type can be targeted using the NG2 promoter. We selected a tetracycline-controllable gene induction system for cell type-specific transgene expression, and generated NG2-tetracycline transactivator (tTA) transgenic lines. We crossed tTA lines with the tetO-ChR2 (channelrhodopsin-2)-EYFP line to characterize tTA-dependent transgene induction. We isolated two unique NG2-tTA mouse lines: one that induced ChR2-EYFP only in mural cells, likely due to the chromosomal position effect of NG2-tTA insertion, and the other that induced it in both cell types. We then applied a Cre-mediated set-subtraction strategy to the latter case and eliminated ChR2-EYFP from mural cells, resulting in NG2 glia-specific transgene induction. We further demonstrated that tTA-dependent ChR2 expression could manipulate cell function. Optogenetic mural cell activation decreased cerebral blood flow, as previously reported, indicating that tTA-mediated ChR2 expression was sufficient to impact cellular function. ChR2-mediated depolarization was observed in NG2 glia in acute hippocampal slices. In addition, ChR2-mediated depolarization of NG2 glia inhibited their proliferation but promoted their differentiation in juvenile mice. Since the tTA-tetO combination is expandable, the mural cell-specific NG2-tTA line and the NG2 glia-specific NG2-tTA line will permit us to conduct observational and manipulation studies to examine in vivo function of these cells separately.

Contact
Kenji F Tanaka , Keio University School of Medicine , Division of Brain Sciences
Contributors
Mitsuhiro OishiMitsuhiro Oishi


Dataset List of 268-Oishi-VesselClassification

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 10988
Dataset Kind Image data
Dataset Size 536.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10989
Dataset Kind Image data
Dataset Size 627.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10990
Dataset Kind Image data
Dataset Size 591.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10991
Dataset Kind Image data
Dataset Size 337.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10992
Dataset Kind Image data
Dataset Size 288.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10993
Dataset Kind Image data
Dataset Size 470.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10994
Dataset Kind Image data
Dataset Size 276.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10995
Dataset Kind Image data
Dataset Size 246.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10996
Dataset Kind Image data
Dataset Size 355.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10997
Dataset Kind Image data
Dataset Size 282.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10998
Dataset Kind Image data
Dataset Size 343.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 10999
Dataset Kind Image data
Dataset Size 331.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11000
Dataset Kind Image data
Dataset Size 561.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11001
Dataset Kind Image data
Dataset Size 539.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11002
Dataset Kind Image data
Dataset Size 450.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11003
Dataset Kind Image data
Dataset Size 276.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11004
Dataset Kind Image data
Dataset Size 324.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11005
Dataset Kind Image data
Dataset Size 397.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11006
Dataset Kind Image data
Dataset Size 375.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11007
Dataset Kind Image data
Dataset Size 331.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11008
Dataset Kind Image data
Dataset Size 379.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data