Summary of 247-Adhitama-Embryogenesis

SSBD:database
SSBD:database URL
Title
Fluorescence images of developmental stages of D. magna during early embryogenesis.
Description
-
Relase date
2022-12-13
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
75.2 MB ( Image datasets: 75.2 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Daphnia magna
Strain(s)
HG-1
Gene symbol(s)
NA
Protein name(s)
H2B, Spo
Protein tag(s)
GFP

Datatype
-
Molecular Function (MF)
Biological Process (BP)
embryo development
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
X scale
84.66582 micrometer/pixel
Y scale
84.66582 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Nikko Adhitama, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe (2020) Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna., PloS one, Volume 15, Number 10, pp. e0239893

Published in 2020 (Electronic publication in Oct. 9, 2020, midnight )

(Abstract) The ecdysteroid and sesquiterpenoid pathways control growth, developmental transition, and embryogenesis in insects. However, the function of orthologous genes and the cross-talk between both pathways remain largely uncharacterized in non-insect arthropods. Spook (Spo) and Juvenile hormone acid o-methyltransferase (Jhamt) have been suggested to function as rate-limiting factors in ecdysteroid and sesquiterpenoid biosynthesis, respectively, in insects. In this study, we report on the functions of Spo and Jhamt and the cross-talk between them in embryos of the branchiopod crustacean Daphnia magna. Spo expression was activated at the onset of gastrulation, with the depletion of Spo transcript by RNAi resulting in developmental arrest at this stage. This phenotype could be partially rescued by supplementation with 20-hydroxyecdysone, indicating that Spo may play the same role in ecdysteroid biosynthesis in early embryos, as reported in insects. After hatching, Spo expression was repressed, while Jhamt expression was activated transiently, despite its silencing during other embryonic stages. Jhamt RNAi showed little effect on survival, but shortened the embryonic period. Exposure to the sesquiterpenoid analog Fenoxycarb extended the embryonic period and rescued the Jhamt RNAi phenotype, demonstrating a previously unidentified role of sesquiterpenoid in the repression of precocious embryogenesis. Interestingly, the knockdown of Jhamt resulted in the derepression of ecdysteroid biosynthesis genes, including Spo, similar to regulation during insect hormonal biosynthesis. Sesquiterpenoid signaling via the Methoprene-tolerant gene was found to be responsible for the repression of ecdysteroid biosynthesis genes. It upregulated an ortholog of CYP18a1 that degrades ecdysteroid in insects. These results illuminate the conserved and specific functions of the ecdysteroid and sesquiterpenoid pathways in Daphnia embryos. We also infer that the common ancestor of branchiopod crustaceans and insects exhibited antagonism between the two endocrine hormones before their divergence 400 million years ago.
(MeSH Terms)

Contact
Hajime Watanabe , Osaka University , Department of Biotechnology, Graduate School of Engineering
Contributors


Dataset List of 247-Adhitama-Embryogenesis

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 8820
Dataset Kind Image data
Dataset Size 27.4 MB
4D view
SSBD:OMERO
Download BDML
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# 8821
Dataset Kind Image data
Dataset Size 15.9 MB
4D view
SSBD:OMERO
Download BDML
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# 8822
Dataset Kind Image data
Dataset Size 15.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 8823
Dataset Kind Image data
Dataset Size 15.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data