Summary of 245-Hirasawa-OR-PAM

SSBD:database
SSBD:database URL
Title
Spectroscopic OR-PAM images of HeLa cells in the planar-culture or dispersed in Matrigel.
Description
-
Relase date
2022-12-13
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
5 ( Image datasets: 5, Quantitative data datasets: 0 )
Size of Datasets
14.8 MB ( Image datasets: 14.8 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell
Gene symbol(s)
NA
Protein name(s)
NA
Reporter(s)
CellBrite NIR680, CellBrite NIR750, CellBrite NIR790, CellBrite Red

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy, photoacoustic microscopy
X scale
2.0 micrometer/pixel, 0.46 micrometer/pixel
Y scale
2.0 micrometer/pixel, 0.46 micrometer/pixel
Z scale
60 micrometer/slice, NA
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Takeshi Hirasawa, Kazuyoshi Tachi, Manami Miyashita, Shinpei Okawa, Toshihiro Kushibiki, Miya Ishihara (2022) Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay., Photoacoustics, Volume 26, pp. 100364

Published in 2022 Jun (Electronic publication in May 7, 2022, midnight )

(Abstract) In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials.

Contact
Miya Ishihara, Takeshi Hirasawa , National Defense Medical College, National Defense Medical College , Department of Medical Engineering, Department of Medical Engineering
Contributors


Dataset List of 245-Hirasawa-OR-PAM

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 8824
Dataset Kind Image data
Dataset Size 276.9 KB
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SSBD:OMERO
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# 8825
Dataset Kind Image data
Dataset Size 10.0 MB
4D view
SSBD:OMERO
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# 8826
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 8827
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 8828
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
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