Summary of 24-Kanemura-iPSCellDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
10.5 MB ( Image datasets: 10.5 MB, Quantitative data datasets: 0 bytes )

Organism(s)
H. sapiens
Strain(s)
iPS
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell
Biological Imaging Method
-
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Phase
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Hoshimi Kanemura, Masahiro J Go, Naoki Nishishita, Noriko Sakai, Hiroyuki Kamao, Yoji Sato, Masayo Takahashi, Shin Kawamata (2013) Pigment epithelium-derived factor secreted from retinal pigment epithelium facilitates apoptotic cell death of iPSC., Scientific reports, Volume 3, pp. 2334

Published in 2013

(Abstract) We show that pigment epithelium-derived factor (PEDF), which is secreted from primary or iPSC-derived retinal pigment epithelium (RPE), dramatically inhibits the growth of iPSCs. PEDF is detected abundantly in culture supernatants of primary or iPSC-derived RPE. Apoptotic cell death is induced in iPSC when co-cultured with RPE, a process that is significantly blocked by addition of antibody against PEDF. Indeed, addition of recombinant PEDF to the iPSC cell culture induces apoptotic cell death in iPSCs, but the expression of pluripotency related-genes is maintained, suggesting that PEDF causes cell death, not differentiation, of iPSCs. To recapitulate this event in vivo, we examined tumor formation in NOG mice after subcutaneous injection of iPSCs with or without an iPSC-derived RPE sheet (2.5 x 10(5) RPE cells). We observed that the tumor forming potential of iPSCs was significantly suppressed by simultaneous transplantation with an iPSC-derived RPE sheet.
(MeSH Terms)

Contact
Shin Kawamata , Foundation for Biomedical Research and Innovation , Research & Development Center for Cell Therapy , Laboratory for Retinal Regeneration
Contributors
Hoshimi Kanemura, Masahiro J. Go, Naoki Nishishita, Noriko Sakai, Hiroyuki Kamao, Yoji Sato, Masayo Takahashi, Shin Kawamata


Dataset List of 24-Kanemura-iPSCellDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 829
Datast ID Fig2B_alone
Dataset Kind Image data
Dataset Size 3.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 830
Datast ID Fig2B_co-cultured
Dataset Kind Image data
Dataset Size 3.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 831
Datast ID Fig3C_IgG
Dataset Kind Image data
Dataset Size 1.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 832
Datast ID Fig3C_PEDF_Ab
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data