Summary of 219-Konishi-CalciumIonDyn

SSBD:database
SSBD:database URL
Title
Live imaging of Ca2+ concentration dynamics in BrafV600E melanoma cells.
Description
-
Relase date
2023-02-21
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
170 ( Image datasets: 170, Quantitative data datasets: 0 )
Size of Datasets
81.4 GB ( Image datasets: 81.4 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus, Canis lupus familiaris
Cell lines(s)
MDCK cell, BrafV600E cell
Gene symbol(s)
Gnaq, Ptger1, Ptgfr, Ptgs1, Ptgs1. Ptgs2. Gnaq, Ptgs2, Tbxa2r, Tbxas1, Tbxas2r
Protein name(s)
NFAT, PKA

Datatype
-
Molecular Function (MF)
G protein-coupled receptor binding
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy, bioluminescence imaging
X scale
NA, 0.441 micrometer/pixel, 0.638924 micrometer/pixel, 0.637 micrometer/pixel, 0.552 micrometer/pixel, 0.828 micrometer/pixel, 0.454 micrometer/pixel, 0.414 micrometer/pixel
Y scale
NA, 0.441 micrometer/pixel, 0.638924 micrometer/pixel, 0.637 micrometer/pixel, 0.552 micrometer/pixel, 0.828 micrometer/pixel, 0.454 micrometer/pixel, 0.414 micrometer/pixel
Z scale
5 micrometer/slice, NA, 1 micrometer/slice
T scale
3.08 sec per time interval, 60.09 sec per time interval, 61.28 sec per time interval, 3.05 sec per time interval, 60.19 sec per time interval, 65.43 sec per time interval, 1.11 sec per time interval, 60.22 sec per time interval, 3.07 sec per time interval, 3 sec per time interval, 97.39 sec per time interval, 60.11 sec per time interval, 6.02 sec per time interval, 6 sec per time interval, 60.73 sec per time interval, 15 sec per time interval, 60.18 sec per time interval, 65.3 sec per time interval, 60.31 sec per time interval, 6.01 sec per time interval, 60.30 sec per time interval, 1 sec per time interval, 3.02 sec per time interval, 3.01 sec per time interval, 5.98 sec per time interval, 3.06 sec per time interval, 1 min per time interval, 60.08 sec per time interval, 3.03 sec per time interval, 59.71 sec per time interval, 3.11 sec per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Yoshinobu Konishi, Hiroshi Ichise, Tetsuya Watabe, Choji Oki, Shinya Tsukiji, Yoko Hamazaki, Yasuhiro Murakawa, Akifumi Takaori-Kondo, Kenta Terai, Michiyuki Matsuda (2021) Intravital Imaging Identifies the VEGF-TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion., Cancer research, Volume 81, Number 15, pp. 4124-4132

Published in 2021 Aug 1 (Electronic publication in May 25, 2021, midnight )

(Abstract) Prostaglandin E2 (PGE2) promotes tumor progression through evasion of antitumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE2, little is known whether PGE2 secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A2 (TXA2) triggers Ca(2+) transients in tumor cells, culminating in PGE2 secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca(2+) transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca(2+) transients were monitored as the surrogate marker of PGE2 secretion. Intravital imaging of Braf(V600E) mouse melanoma cells revealed that the proportion of cells exhibiting Ca(2+) transients is markedly higher in vivo than in vitro. The TXA2 receptor was indispensable for the Ca(2+) transients in vivo, high intratumoral PGE2 concentration, and evasion of antitumor immunity. Notably, treatment with a VEGF receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca(2+) transients and reduced TXA2 and PGE2 concentrations in tumor tissues. These results identify the VEGF-TXA2 axis as a critical promoter of PGE2-dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies. SIGNIFICANCE: This study identifies the VEGF-TXA2 axis as a potentially targetable regulator of PGE2 secretion, which provides novel strategies for prevention and treatment of multiple types of malignancies.
(MeSH Terms)

Contact
Kenta Terai , kyoto University , Graduate School of Medicine , Department of Pathology and Biology of Diseases
Contributors


Dataset List of 219-Konishi-CalciumIonDyn

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Dataset ID
Kind
Size
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# 10319
Dataset Kind Image data
Dataset Size 2.0 GB
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# 10320
Dataset Kind Image data
Dataset Size 1.8 GB
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# 10321
Dataset Kind Image data
Dataset Size 303.1 MB
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# 10322
Dataset Kind Image data
Dataset Size 302.4 MB
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# 10323
Dataset Kind Image data
Dataset Size 302.4 MB
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# 10324
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10325
Dataset Kind Image data
Dataset Size 420.7 MB
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# 10326
Dataset Kind Image data
Dataset Size 926.0 MB
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# 10327
Dataset Kind Image data
Dataset Size 927.0 MB
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# 10328
Dataset Kind Image data
Dataset Size 821.8 MB
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# 10329
Dataset Kind Image data
Dataset Size 455.8 MB
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# 10330
Dataset Kind Image data
Dataset Size 455.9 MB
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# 10331
Dataset Kind Image data
Dataset Size 455.8 MB
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# 10332
Dataset Kind Image data
Dataset Size 926.0 MB
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# 10333
Dataset Kind Image data
Dataset Size 926.0 MB
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# 10334
Dataset Kind Image data
Dataset Size 1.1 GB
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# 10335
Dataset Kind Image data
Dataset Size 927.6 MB
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# 10336
Dataset Kind Image data
Dataset Size 927.6 MB
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# 10337
Dataset Kind Image data
Dataset Size 927.6 MB
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SSBD:OMERO
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# 10338
Dataset Kind Image data
Dataset Size 213.8 MB
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SSBD:OMERO
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# 10339
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10340
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10341
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10342
Datast ID Figure1E_GnaqKO_N1
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10343
Datast ID Figure1E_GnaqKO_N2
Dataset Kind Image data
Dataset Size 412.8 MB
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SSBD:OMERO
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# 10344
Datast ID Figure1E_GnaqKO_N3
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10345
Dataset Kind Image data
Dataset Size 6.2 MB
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SSBD:OMERO
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# 10346
Dataset Kind Image data
Dataset Size 19.9 MB
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SSBD:OMERO
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# 10347
Dataset Kind Image data
Dataset Size 12.6 MB
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SSBD:OMERO
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# 10348
Dataset Kind Image data
Dataset Size 12.6 MB
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SSBD:OMERO
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# 10349
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10350
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10351
Dataset Kind Image data
Dataset Size 412.3 MB
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SSBD:OMERO
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# 10352
Dataset Kind Image data
Dataset Size 1.5 GB
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SSBD:OMERO
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# 10353
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10354
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10355
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10356
Dataset Kind Image data
Dataset Size 97.8 MB
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SSBD:OMERO
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# 10357
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10358
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10359
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10360
Dataset Kind Image data
Dataset Size 413.4 MB
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SSBD:OMERO
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# 10361
Dataset Kind Image data
Dataset Size 432.2 MB
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SSBD:OMERO
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# 10362
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10363
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10364
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10365
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10366
Dataset Kind Image data
Dataset Size 781.4 MB
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# 10367
Dataset Kind Image data
Dataset Size 514.3 MB
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# 10368
Datast ID Figure4B_VEGFRi_N1
Dataset Kind Image data
Dataset Size 405.9 MB
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# 10369
Datast ID Figure4B_VEGFRi_N2
Dataset Kind Image data
Dataset Size 202.9 MB
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# 10370
Datast ID Figure4B_VEGFRi_N3
Dataset Kind Image data
Dataset Size 202.9 MB
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# 10371
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10372
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10373
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10374
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10375
Dataset Kind Image data
Dataset Size 155.4 MB
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# 10376
Dataset Kind Image data
Dataset Size 155.4 MB
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# 10377
Dataset Kind Image data
Dataset Size 1.8 GB
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# 10378
Dataset Kind Image data
Dataset Size 1.5 GB
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# 10379
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10380
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10381
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10382
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10383
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10384
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10385
Dataset Kind Image data
Dataset Size 1.8 GB
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# 10386
Dataset Kind Image data
Dataset Size 31.9 MB
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# 10387
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10388
Dataset Kind Image data
Dataset Size 412.3 MB
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# 10389
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10390
Dataset Kind Image data
Dataset Size 414.8 MB
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# 10391
Dataset Kind Image data
Dataset Size 416.9 MB
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# 10392
Dataset Kind Image data
Dataset Size 415.8 MB
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# 10393
Dataset Kind Image data
Dataset Size 1.8 GB
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# 10394
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10395
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10396
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10397
Dataset Kind Image data
Dataset Size 432.2 MB
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# 10398
Dataset Kind Image data
Dataset Size 426.1 MB
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SSBD:OMERO
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# 10399
Dataset Kind Image data
Dataset Size 432.2 MB
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SSBD:OMERO
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# 10400
Datast ID Figure5D_Gnaq_KO
Dataset Kind Image data
Dataset Size 8.7 MB
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SSBD:OMERO
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# 10401
Dataset Kind Image data
Dataset Size 8.4 MB
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# 10402
Dataset Kind Image data
Dataset Size 11.2 MB
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# 10403
Dataset Kind Image data
Dataset Size 1.5 GB
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# 10404
Dataset Kind Image data
Dataset Size 141.6 MB
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# 10405
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10406
Dataset Kind Image data
Dataset Size 190.0 MB
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# 10407
Dataset Kind Image data
Dataset Size 190.0 MB
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SSBD:OMERO
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# 10408
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10409
Dataset Kind Image data
Dataset Size 1004.0 MB
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# 10410
Dataset Kind Image data
Dataset Size 51.6 MB
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# 10411
Dataset Kind Image data
Dataset Size 292.8 MB
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# 10412
Dataset Kind Image data
Dataset Size 567.2 MB
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# 10413
Dataset Kind Image data
Dataset Size 283.6 MB
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# 10414
Dataset Kind Image data
Dataset Size 105.2 MB
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# 10415
Dataset Kind Image data
Dataset Size 104.2 MB
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# 10416
Dataset Kind Image data
Dataset Size 206.7 MB
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# 10417
Dataset Kind Image data
Dataset Size 31.9 MB
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# 10418
Dataset Kind Image data
Dataset Size 616.8 MB
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# 10419
Dataset Kind Image data
Dataset Size 616.8 MB
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# 10420
Dataset Kind Image data
Dataset Size 821.8 MB
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# 10421
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10422
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10423
Dataset Kind Image data
Dataset Size 411.7 MB
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# 10424
Dataset Kind Image data
Dataset Size 143.9 MB
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# 10425
Dataset Kind Image data
Dataset Size 309.2 MB
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# 10426
Dataset Kind Image data
Dataset Size 207.7 MB
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# 10427
Dataset Kind Image data
Dataset Size 821.8 MB
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# 10428
Dataset Kind Image data
Dataset Size 2.1 GB
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# 10429
Dataset Kind Image data
Dataset Size 1.8 GB
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# 10430
Dataset Kind Image data
Dataset Size 1.7 GB
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# 10431
Dataset Kind Image data
Dataset Size 608.9 MB
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# 10432
Dataset Kind Image data
Dataset Size 608.9 MB
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# 10433
Dataset Kind Image data
Dataset Size 710.4 MB
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SSBD:OMERO
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# 10434
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10435
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10436
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10437
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10438
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10439
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10440
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10441
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10442
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10443
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10444
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10445
Dataset Kind Image data
Dataset Size 435.4 MB
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# 10446
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10447
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10448
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10449
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10450
Dataset Kind Image data
Dataset Size 435.4 MB
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SSBD:OMERO
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# 10451
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10452
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10453
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10454
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10455
Datast ID SuppFig5B_WT1_pre
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10456
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10457
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10458
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10459
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10460
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10461
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10462
Datast ID SuppFig5B_WT2_pre
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10463
Dataset Kind Image data
Dataset Size 217.7 MB
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SSBD:OMERO
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# 10464
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10465
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10466
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10467
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10468
Datast ID SuppFig5B_WT3_pre
Dataset Kind Image data
Dataset Size 217.7 MB
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# 10469
Datast ID SuppFig6CD_Ly6G_N1
Dataset Kind Image data
Dataset Size 2.9 GB
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SSBD:OMERO
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# 10470
Datast ID SuppFig6CD_Ly6G_N2
Dataset Kind Image data
Dataset Size 310.2 MB
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SSBD:OMERO
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# 10471
Datast ID SuppFig6CD_Ly6G_N3
Dataset Kind Image data
Dataset Size 310.2 MB
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SSBD:OMERO
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# 10472
Dataset Kind Image data
Dataset Size 372.3 MB
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SSBD:OMERO
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# 10473
Dataset Kind Image data
Dataset Size 125.9 MB
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SSBD:OMERO
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# 10474
Dataset Kind Image data
Dataset Size 122.8 MB
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SSBD:OMERO
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# 10475
Dataset Kind Image data
Dataset Size 515.9 MB
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SSBD:OMERO
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# 10476
Dataset Kind Image data
Dataset Size 1.2 GB
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SSBD:OMERO
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# 10477
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10478
Dataset Kind Image data
Dataset Size 309.2 MB
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SSBD:OMERO
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# 10479
Dataset Kind Image data
Dataset Size 627.7 MB
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SSBD:OMERO
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# 10480
Dataset Kind Image data
Dataset Size 141.6 MB
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SSBD:OMERO
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# 10481
Dataset Kind Image data
Dataset Size 143.9 MB
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SSBD:OMERO
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# 10482
Dataset Kind Image data
Dataset Size 411.7 MB
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SSBD:OMERO
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# 10483
Dataset Kind Image data
Dataset Size 411.7 MB
4D view
SSBD:OMERO
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# 10484
Dataset Kind Image data
Dataset Size 411.7 MB
4D view
SSBD:OMERO
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# 10485
Dataset Kind Image data
Dataset Size 411.7 MB
4D view
SSBD:OMERO
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# 10486
Dataset Kind Image data
Dataset Size 1.2 GB
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SSBD:OMERO
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# 10487
Dataset Kind Image data
Dataset Size 1.1 GB
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SSBD:OMERO
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# 10488
Dataset Kind Image data
Dataset Size 2.1 GB
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SSBD:OMERO
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